Analysis Tools
cellular
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• at E3.0, all morula embryos exhibit many TUNEL+ blastomeres, indicating increased DNA damage and cell death
• however, no obvious TRP53+ cells are detected at E3.0, suggesting that cell death is not TRP53dependent
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embryo
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• at E3.0, all morula embryos exhibit many TUNEL+ blastomeres, indicating increased DNA damage and cell death
• however, no obvious TRP53+ cells are detected at E3.0, suggesting that cell death is not TRP53dependent
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• embryos fail to reach a morphological blastocyst stage after 3 days in culture
(J:279207)
• none of the embryos form blastocysts in 3-day outgrowth assays
(J:299155)
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• in culture, embryos fail to hatch from the zona pellucida with apparent cell death, never reaching a morphological blastocyst stage
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• embryos show no further development with apparent cell death after 3 days in culture
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• at E3.5, all embryos exhibit a compacted morula morphology rather than differentiation into blastocysts, indicating delayed development
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• after 3 days in culture, all embryos show a necrotic/lysed morphology still trapped inside the zona pellucida
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• at E3.5, none of the embryos exhibit signs of cavitation
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• at E3.0, immunofluorescence staining of CDX2 (trophectoderm marker) is strikingly reduced, suggesting impaired trophectoderm specification
• in contrast, no change in OCT4 expression and inner cell mass (ICM) lineage development is observed
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growth/size/body
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• at E3.5, all embryos exhibit a compacted morula morphology rather than differentiation into blastocysts, indicating delayed development
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mortality/aging
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IMPC - JAX
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IMPC - JAX
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• homozygous embryos appearing as compacted morulas are recovered in expected Mendelian ratios at E3.5 but arrest/die prior to blastocyst formation; no embryos or excess resorption sites are observed at E7.5
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reproductive system
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• no embryos or empty decidua are found at E7.5, suggesting failure of embryo implantation
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