embryo
• at E6.5, embryos show a complete loss of Oct4, Nanog and Sox2 mRNA as well as protein, indicating loss of critical pluripotency factors
• embryos exhibit disorganization by E7.5
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• embryos successfully implant and form egg-cylinder stages but fail to initiate gastrulation; no features of gastrulating embryos are noted at E6.5 or E7.5
• expression of brachyury (T) is almost indetectable at E6.5, indicating a deficiency in gastrulation initiation
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• embryos reach egg-cylinder stage but do not initiate gastrulation
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• embryos are significantly delayed at E6.5 and E7.5
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• embryos are significantly smaller than controls at E6.5 and E7.5
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• at E6.5, expression of OCT4 and NANOG (epiblast markers) is completely absent while expression of CDX2 (a marker of extraembryonic ectoderm) is normal, indicating loss of epiblast pluripotency in pre-streak stage embryos
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• epiblast is markedly reduced in size at E6.5
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• no early head folds are present at E6.5 or E7.5
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• no embryonic node is present at E6.5 or E7.5
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• no morphological evidence of primitive streak formation is observed at E6.5 or E7.5
• expression of brachyury (T) is almost indetectable at E6.5, indicating a deficiency in primitive streak formation
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• no allantois is identified at E6.5 or E7.5
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• no amnion is identified at E6.5 or E7.5
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• no chorion is identified at E6.5 or E7.5
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• extraembryonic ectoderm is markedly reduced in size at E6.5
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• pro-amniotic cavity is markedly reduced in size at E6.5
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growth/size/body
• embryos are significantly delayed at E6.5 and E7.5
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• embryos are significantly smaller than controls at E6.5 and E7.5
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mortality/aging
IMPC - JAX
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• although embryos are present at Mendelian ratios and appear morphologically normal at E3.5, only 12% of embryos are recovered from heterozygous intercrosses at E6.5 and E7.5
• no embryos are identified at E9.5 or later stages
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IMPC - JAX
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reproductive system
• 9 of 93 (~10%) decidua from heterozygous crosses are empty at E6.5 and E7.5
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