Analysis Tools
embryo
|
• at E6.5, embryos show a complete loss of Oct4, Nanog and Sox2 mRNA as well as protein, indicating loss of critical pluripotency factors
• embryos exhibit disorganization by E7.5
|
|
• no morphological hallmarks typical of gastrulating embryos are observed at E7.5
(J:279207)
• no Brachyury is expressed at E6.5, suggesting failure to initiate gastrulation
(J:279207)
• embryos successfully implant and form egg-cylinder stages but fail to initiate gastrulation; no features of gastrulating embryos are noted at E6.5 or E7.5
(J:348275)
• expression of brachyury (T) is almost indetectable at E6.5, indicating a deficiency in gastrulation initiation
(J:348275)
|
|
• embryos reach egg-cylinder stage but do not initiate gastrulation
|
|
• embryos are significantly delayed at E6.5 and E7.5
|
|
• embryos are much smaller than controls at E6.5 and E7.5
(J:279207)
• embryos are significantly smaller than controls at E6.5 and E7.5
(J:348275)
|
|
• at E6.5, expression of OCT4 and NANOG (epiblast markers) is completely absent while expression of CDX2 (a marker of extraembryonic ectoderm) is normal, indicating loss of epiblast pluripotency in pre-streak stage embryos
|
|
• epiblast is markedly reduced in size at E6.5
|
|
• a rudimentary egg cylinder is observed at E7.5
|
|
• no head folds are present at E7.5
(J:279207)
• no early head folds are present at E6.5 or E7.5
(J:348275)
|
|
• no embryonic node is present at E7.5
(J:279207)
• no embryonic node is present at E6.5 or E7.5
(J:348275)
|
|
• no morphological primitive streak formation is present at E7.5
(J:279207)
• no morphological evidence of primitive streak formation is observed at E6.5 or E7.5
(J:348275)
• expression of brachyury (T) is almost indetectable at E6.5, indicating a deficiency in primitive streak formation
(J:348275)
|
|
• no allantois is identified at E6.5 or E7.5
|
|
• no amnion is identified at E6.5 or E7.5
|
|
• no chorion is identified at E6.5 or E7.5
|
|
• extraembryonic ectoderm is markedly reduced in size at E6.5
|
|
• pro-amniotic cavity is markedly reduced in size at E6.5
|
growth/size/body
|
• embryos are significantly delayed at E6.5 and E7.5
|
|
• embryos are much smaller than controls at E6.5 and E7.5
(J:279207)
• embryos are significantly smaller than controls at E6.5 and E7.5
(J:348275)
|
mortality/aging
|
IMPC - JAX
|
|
IMPC - JAX
|
|
• homozygous embryos can be recovered at E7.5 (13.5% versus expected 25%, n=81) but arrest prior to gastrulation
(J:279207)
• although embryos are present at Mendelian ratios and appear morphologically normal at E3.5, only 12% of embryos are recovered from heterozygous intercrosses at E6.5 and E7.5; no embryos are identified at E9.5 or later stages
(J:348275)
|
reproductive system
|
• 9 of 93 (~10%) decidua from heterozygous crosses are empty at E6.5 and E7.5
|
