All Phenotypes Taf13<em1(IMPC)J> MGI Mouse

abnormal embryo development ( J:348275 )

• at E6.5, embryos show a complete loss of Oct4, Nanog and Sox2 mRNA as well as protein, indicating loss of critical pluripotency factors

• embryos exhibit disorganization by E7.5

failure to gastrulate ( J:348275 )

• embryos successfully implant and form egg-cylinder stages but fail to initiate gastrulation; no features of gastrulating embryos are noted at E6.5 or E7.5

• expression of brachyury (T) is almost indetectable at E6.5, indicating a deficiency in gastrulation initiation

embryonic growth arrest ( J:348275 )

• embryos reach egg-cylinder stage but do not initiate gastrulation

embryonic growth retardation ( J:348275 )

• embryos are significantly delayed at E6.5 and E7.5

decreased embryo size ( J:348275 )

• embryos are significantly smaller than controls at E6.5 and E7.5

abnormal embryonic epiblast morphology ( J:348275 )

• at E6.5, expression of OCT4 and NANOG (epiblast markers) is completely absent while expression of CDX2 (a marker of extraembryonic ectoderm) is normal, indicating loss of epiblast pluripotency in pre-streak stage embryos

small embryonic epiblast ( J:348275 )

• epiblast is markedly reduced in size at E6.5

absent head fold ( J:348275 )

• no early head folds are present at E6.5 or E7.5

absent primitive node ( J:348275 )

• no embryonic node is present at E6.5 or E7.5

failure of primitive streak formation ( J:348275 )

• no morphological evidence of primitive streak formation is observed at E6.5 or E7.5

• expression of brachyury (T) is almost indetectable at E6.5, indicating a deficiency in primitive streak formation

absent allantois ( J:348275 )

• no allantois is identified at E6.5 or E7.5

absent amnion ( J:348275 )

• no amnion is identified at E6.5 or E7.5

absent chorion ( J:348275 )

• no chorion is identified at E6.5 or E7.5

abnormal extraembryonic ectoderm morphology ( J:348275 )

• extraembryonic ectoderm is markedly reduced in size at E6.5

small proamniotic cavity ( J:348275 )

• pro-amniotic cavity is markedly reduced in size at E6.5

embryonic growth retardation ( J:348275 )

• embryos are significantly delayed at E6.5 and E7.5

decreased embryo size ( J:348275 )

• embryos are significantly smaller than controls at E6.5 and E7.5

embryonic lethality prior to organogenesis ( J:211773 )

IMPC - JAX

embryonic lethality prior to organogenesis, complete penetrance ( J:348275 )

• although embryos are present at Mendelian ratios and appear morphologically normal at E3.5, only 12% of embryos are recovered from heterozygous intercrosses at E6.5 and E7.5

• no embryos are identified at E9.5 or later stages

preweaning lethality, complete penetrance ( J:211773 )

IMPC - JAX

empty decidua capsularis ( J:348275 )

• 9 of 93 (~10%) decidua from heterozygous crosses are empty at E6.5 and E7.5

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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