Phenotypes associated with this allele

• Allele Symbol: Klf14^em1Cgw
• Allele Name: endonuclease-mediated mutation 1, Chuangui Wang
• Allele ID: MGI:5907263
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Klf14^em1Cgw/Klf14^em1Cgw	C57BL/6J-Klf14^em1Cgw	MGI:5907265

Genotype
MGI:5907265

hm1

• Allelic Composition: Klf14^em1Cgw/Klf14^em1Cgw
• Genetic Background: C57BL/6J-Klf14^em1Cgw

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

neoplasm

increased tumor incidence ( J:227200 )

• increase in incidence starting at about 11 months of age

• incidence reaches 33.3% at 13-14 months of age

increased incidence of tumors by chemical induction ( J:227200 )

• following an azoxymethane and dextran sulfate sodium tumor induction protocol null mice developed more, larger, and higher grade adenomas in the colon

increased lung adenoma incidence ( J:227200 )

• in 2 of 27 mice

• adenomas are relatively spherical lesions with discrete borders and composed of basophilic tumor cells uniform in appearance

increased lymphoma incidence ( J:227200 )

• lymphoma in the spleen and lymph nodes in 4 and 3 of 27 mice, respectively

• lymphomas are characterized by the Reed/Sternberg cells or large cells with horse-shoe-shaped nuclei surrounded by lightly staining eosinophilic area

cellular

abnormal centrosome morphology ( J:227200 )

• increased incidence of centrosome amplification in both interphase and mitotic MEFs

aneuploidy ( J:227200 )

• about 20% of MEFs at passage 3 are aneuploid compared to less than 4% of wild-type cells

polyploidy ( J:227200 )

• MEFs are found with greater than 4N DNA content at passage 3

abnormal mitosis ( J:227200 )

• greater than 16% of mitotic passage 3 MEFs display chromosome missegregation compared to only about 3% of wild-type cells

chromosomal instability ( J:227200 )

• genome instability in passage 3 MEFs

homeostasis/metabolism

increased incidence of tumors by chemical induction ( J:227200 )

• following an azoxymethane and dextran sulfate sodium tumor induction protocol null mice developed more, larger, and higher grade adenomas in the colon

respiratory system

increased lung adenoma incidence ( J:227200 )

• in 2 of 27 mice

• adenomas are relatively spherical lesions with discrete borders and composed of basophilic tumor cells uniform in appearance