Phenotypes associated with this allele

• Allele Symbol: Mcm3^tm1.1Geno
• Allele Name: targeted mutation 1.1, Genoway
• Allele ID: MGI:5907491
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mcm3^tm1.1Geno/Mcm3^tm1.1Geno	involves: 129S2/SvPas * C57BL/6	MGI:5907493
hm2	Mcm3^tm1.1Geno/Mcm3^tm1.1Geno	involves: 129S2/SvPas * C57BL/6 * CD-1	MGI:5907495
ht3	Mcm3^tm1.1Geno/Mcm3^+	involves: 129S2/SvPas * C57BL/6	MGI:5907494
cx4	Mcm3^tm1.1Geno/Mcm3^tm1.1Geno Tg(Chek1)1Ofc/0	involves: 129S2/SvPas * C57BL/6 * CD-1	MGI:5907496

Genotype
MGI:5907493

hm1

• Allelic Composition: Mcm3^tm1.1Geno/Mcm3^tm1.1Geno
• Genetic Background: involves: 129S2/SvPas * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

perinatal lethality, incomplete penetrance ( J:227638 )

• survive until E16.5-E18.5 but only 1 out of 314 mice produced was homozygous

• Background Sensitivity: survival to birth is decreased compared to homozygous mice on a mixed background that includes CD-1

hematopoietic system

decreased hematopoietic stem cell proliferation ( J:227638 )

• decreased proliferation potential

abnormal proerythroblast morphology ( J:227638 )

• accumulation of pro- and early basophilic erythroblasts in the fetal liver and reduction in the number of chromatophilic/orthochromatophilic erythroblasts and mature reticulocytes

increased erythroblast number ( J:227638 )

• large accumulation of erythroblasts with uncompacted or micronuclei in the peripheral blood at E14.5

abnormal erythrocyte morphology ( J:227638 )

abnormal erythropoiesis ( J:227638 )

• impaired erythrocyte maturation in transplanted cells derived from E14.5 fetal livers

decreased fetal derived definitive erythrocyte cell number ( J:227638 )

• marked reduction in Ter119 positive cells in the fetal liver and in red blood cells in the peripheral blood at E14.5

decreased hematocrit ( J:227638 )

• in the peripheral blood at E14.5

decreased hemoglobin content ( J:227638 )

• in the peripheral blood at E14.5

abnormal reticulocyte cell number ( J:227638 )

• decrease in the number of reticulocytes in the fetal liver

increased hematopoietic stem cell number ( J:227638 )

• accumulation of multipotent progenitors

growth/size/body

decreased fetal size ( J:227638 )

• by E14.5-E18.6

liver/biliary system

abnormal liver physiology ( J:227638 )

• increase in the presence of markers of DNA damage in the fetal liver at E16.5

integument

pallor ( J:227638 )

• at E16.5-E18.6, appear pale

cellular

abnormal cell cycle ( J:227638 )

• basophilic and chromatophilic erythroblasts display an accumulation of cells in S phase suggesting a defect in DNA replication

increased apoptosis ( J:227638 )

• apoptotic erythroblasts are seen in the fetal liver

decreased hematopoietic stem cell proliferation ( J:227638 )

• decreased proliferation potential

decreased DNA replication ( J:227638 )

• basophilic and chromatophilic erythroblasts display an accumulation of cells in S phase suggesting a defect in DNA replication

• high levels of markers for stalled fork replication are seen in early erythroblasts

homeostasis/metabolism

decreased DNA replication ( J:227638 )

• basophilic and chromatophilic erythroblasts display an accumulation of cells in S phase suggesting a defect in DNA replication

• high levels of markers for stalled fork replication are seen in early erythroblasts

Genotype
MGI:5907495

hm2

• Allelic Composition: Mcm3^tm1.1Geno/Mcm3^tm1.1Geno
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * CD-1

mortality/aging

prenatal lethality, incomplete penetrance ( J:227638 )

• Background Sensitivity: increased survival compared to mice on a background lacking CD-1, a few mice survive to birth (2.8% compared to the expected 12.5%)

Genotype
MGI:5907494

ht3

• Allelic Composition: Mcm3^tm1.1Geno/Mcm3⁺
• Genetic Background: involves: 129S2/SvPas * C57BL/6

mortality/aging

premature death ( J:227638 )

• slight but significant reduction in lifespan

neoplasm

increased metastatic potential ( J:227638 )

• increased invasiveness of lymphomas

increased tumor incidence ( J:227638 )

• mainly lymphomas originating in the mesenteric lymph nodes that in multiple cases infiltrate to other organs

increased lymphoma incidence ( J:227638 )

• earlier onset and increased invasiveness

decreased tumor latency ( J:227638 )

• of lymphomas

Genotype
MGI:5907496

cx4

• Allelic Composition: Mcm3^tm1.1Geno/Mcm3^tm1.1Geno; Tg(Chek1)1Ofc/0
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * CD-1

mortality/aging

prenatal lethality, incomplete penetrance ( J:227638 )

• partial rescue of the prenatal lethality (7.6% compared to the expected 12.5%)