Phenotypes associated with this allele

• Allele Symbol: Serpina3k^em1(IMPC)J
• Allele Name: endonuclease-mediated mutation 1, Jackson
• Allele ID: MGI:6107619
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Serpina3k^em1(IMPC)J/Serpina3k^em1(IMPC)J	C57BL/6NJ-Serpina3k^em1(IMPC)J/Mmjax	MGI:7764412

Genotype
MGI:7764412

hm1

• Allelic Composition: Serpina3k^em1(IMPC)J/Serpina3k^em1(IMPC)J
• Genetic Background: C57BL/6NJ-Serpina3k^em1(IMPC)J/Mmjax

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

mortality/aging ( J:345330 )

N • mice exhibit a normal survival rate up to at least 18 months of age

renal/urinary system

decreased susceptibility to kidney reperfusion injury ( J:345330 )

♂ • 24 h after induction of bilateral renal ischemia/reperfusion (I/R), males show milder renal dysfunction with a lower elevation of serum creatinine and BUN levels, and a smaller reduction in glomerular filtration rate (GRF) than wild-type controls (53.9 % versus 76.4%, respectively) but with similar increases in acute tubular injury (ATI) and acute tubular necrosis (ATN) scores in the kidney cortex and medulla

♂ • however, after ischemic injury, left and right kidney weights and fractional excretion of sodium are similarly increased in both genotypes

homeostasis/metabolism

decreased circulating creatinine level ( J:345330 )

♂ • 24 h after induction of bilateral renal I/R, serum creatinine levels are significantly lower than in wild-type controls

decreased blood urea nitrogen level ( J:345330 )

♂ • 24 h after induction of bilateral renal I/R, blood urea nitrogen (BUN) levels are significantly lower than in wild-type controls

abnormal physiological response to hypoxia ( J:345330 )

♂ • 24 h after induction of bilateral renal I/R, the expected increase in pro-apoptotic protein BAX levels and downregulation of Hif1a and Vegfa mRNA levels is not observed, suggesting enhanced cellular responses to hypoxic injury

decreased susceptibility to kidney reperfusion injury ( J:345330 )

♂ • 24 h after induction of bilateral renal ischemia/reperfusion (I/R), males show milder renal dysfunction with a lower elevation of serum creatinine and BUN levels, and a smaller reduction in glomerular filtration rate (GRF) than wild-type controls (53.9 % versus 76.4%, respectively) but with similar increases in acute tubular injury (ATI) and acute tubular necrosis (ATN) scores in the kidney cortex and medulla

♂ • however, after ischemic injury, left and right kidney weights and fractional excretion of sodium are similarly increased in both genotypes

cellular

decreased apoptosis ( J:345330 )

♂ • 24 h after induction of bilateral renal I/R, no significant increase in the pro-apoptotic protein BAX (BCL2-associated X protein) is observed, unlike in wild-type controls

abnormal redox activity ( J:345330 )

♂ • under basal (sham surgery) conditions, urinary hydrogen peroxide excretion (UH2O2) and FOXO3 (forkhead box O3) protein levels are significantly higher than those in sham-operated wild-type controls

♂ • after induction of renal I/R, no significant increase in UH2O2 or in SIRT1 (sirtuin 1) protein levels is observed while mRNA levels of the antioxidant enzymes Cat (catalase), Sod2 (superoxide dismutase 2, mitochondrial), and Gpx1(glutathione peroxidase 1) are not downregulated, unlike in I/R wild-type controls

♂ • no change in FOXO3 expression is noted when both genotypes undergo I/R