Phenotypes associated with this allele

• Allele Symbol: Med28^tm1.1Vra
• Allele Name: targeted mutation 1.1, Vijaya Ramesh
• Allele ID: MGI:6111945
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Med28^tm1.1Vra/Med28^tm1.1Vra	involves: 129S4/SvJae * C57BL/6	MGI:6112019
cn2	Med28^tm1.1Vra/Med28^+	involves: 129S4/SvJae * C57BL/6	MGI:6112020

Genotype
MGI:6112019

cn1

• Allelic Composition: Med28^tm1.1Vra/Med28^tm1.1Vra
• Genetic Background: involves: 129S4/SvJae * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

embryo

abnormal pluripotent precursor cell morphology ( J:243694 )

• primary mouse embryonic fibroblasts from E13.4 embryos infected with an adenovirus expressing Cre recombinase (Ad-Cre) are unable to generate induced pluripotent stem cell (iPSC) colonies indicating a failure to reprogram

• induced pluripotent stem cells infected with Ad-Cre fail to survive

Genotype
MGI:6112020

cn2

• Allelic Composition: Med28^tm1.1Vra/Med28⁺
• Genetic Background: involves: 129S4/SvJae * C57BL/6

embryo

abnormal pluripotent precursor cell morphology ( J:243694 )

• induced pluripotent stem cells (iPSC) infected with an adenovirus expressing Cre recombinase (Ad-Cre) exhibit differentiated morphology (into extraembryonic trophectoderm and primitive endoderm lineages) unlike wild-type iPSC that show undifferentiated morphology, indicating loss of induced pluripotency