Phenotypes associated with this allele

• Allele Symbol: Dennd1b^tm1.2Achn
• Allele Name: targeted mutation 1.2, Andrew C Chan
• Allele ID: MGI:6113476
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Dennd1b^tm1.2Achn/Dennd1b^tm1.2Achn	involves: C57BL/6	MGI:6115545

Genotype
MGI:6115545

hm1

• Allelic Composition: Dennd1b^tm1.2Achn/Dennd1b^tm1.2Achn
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:229307 )

N • immune cell numbers at age 5 weeks to 7 months

N • dendritic cell and subset cell numbers in spleen and lymph node

N • dendritic cell activation status in spleen and lymph node

N • splenic dendritic cell antigen presentation to CD8+ OT-I and CD4+ OT-II TCR transgenic T cells in vitro

N • TCR-mediated activation of naive T cells

N • surface levels of CD69 and CD25 upon stimulation with anti-CD3/CD28 mAbs in naive splenic T cells

N • IL-2 production upon stimulation with anti-CD3/CD28 mAbs in naive splenic T cells

N • increases in naive T cell numbers under TH polarizing conditions

N • levels of CD3 in differentiated TH cells

N • IFN-gamma level in TH1 cells following crosslinking with anti-CD3/CD28 mAbs

N • no changes in TH0, TH1 and TH17 cells following OVA antigen presentation by dendritic cells

N • no changes in TCR down-modulation after receptor activation in TH1 and TH17 cells

N • internalization of transferrin receptor after receptor activation in TH2 cells

N • no changes in TCR down-modulation in TH0, TH1 and TH17 cells following activation by anti-CD3/28 mAbs or peptide + antigen-presenting cells

N • unchanged IFN-gamma production in ex vivo recall response of BAL fluid and mediastinal lymph node lymphocytes from OVA challenged mice

abnormal T cell number ( J:229307 )

• more splenic and lymph node CD4+/CD8+ effector/memory T cells in mice older than 7 months

abnormal T-helper 2 physiology ( J:229307 )

• delayed TCR down-modulation following receptor activation

lymph node hyperplasia ( J:229307 )

• increased cell numbers in mediastinal lymph nodes after TNP-OVA immunization

abnormal interleukin secretion ( J:229307 )

• greater than 3-fold increases in IL-4, IL-5, and IL-13 cytokine secretion in TH2 cells following crosslinking with anti-CD3/CD28 mAbs

• increased secretion of IL-4, IL-5, and IL-13 in TH2 cells expressing OT-II TCR transgene specific for OVA ovalbumin after OVA antigen presentation by dendritic cells

• increased IL-4, IL-5 and IL-13 secretion in ex vivo recall response of BAL fluid and mediastinal lymph node lymphocytes from OVA challenged mice

• IL-4, IL-5 and IL-13 secretion in ex vivo recall response of splenic lymphocytes from OVA challenged mice

• IL-17 level in TH17 and IL-2 level in TH1, TH2, TH17 and TH0 cells following crosslinking with anti-CD3/CD28 mAbs

• IL-2 levels in any differentiated T cells following OVA antigen presentation by dendritic cells

• IL-2 levels in ex vivo re-stimulation response of TH2 cells from OVA challenged mice

respiratory system

abnormal lung morphology ( J:229307 )

• increased number of lymphocytes, macrophages and eosinophils in BAL fluid after TNP-OVA immunization

• increased numbers of eosinophils, neutrophils, mast cells, macrophages, dendritic cells, B cells and CD4+ effector/memory T cells in lung tissue after OVA challenge

• increased TNP-OVA-specific IgE and IgG1 levels in BAL and serum after OVA challenge

• number or CD86 activation status of dendritic cell subsets in mediastinal lymph node, lung tissue and spleen after TNP-OVA immunization

enlarged lung ( J:229307 )

• increased cell numbers in lungs and bronchoalveolar lavage (BAL) fluid after TNP-OVA immunization

hematopoietic system

abnormal T cell number ( J:229307 )

• more splenic and lymph node CD4+/CD8+ effector/memory T cells in mice older than 7 months

abnormal T-helper 2 physiology ( J:229307 )

• delayed TCR down-modulation following receptor activation

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:229307 )

N • cellular composition of thymocytes and bone marrow at age 5 weeks to 7 months

reproductive system

reproductive system phenotype ( J:229307 )

N • fertile

growth/size/body

enlarged lung ( J:229307 )

• increased cell numbers in lungs and bronchoalveolar lavage (BAL) fluid after TNP-OVA immunization