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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Pknox1tm1.1Rygo
targeted mutation 1.1, Ryo Goitsuka
MGI:6116700
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Gt(ROSA)26Sortm1(cre/ERT2)Thl/Gt(ROSA)26Sor+
Pknox1tm1.1Rygo/Pknox1tm1.1Rygo 		involves: 129 * C57BL/6 * C57BL/6JJcl MGI:6887744
cn2
 		Alpltm1(cre)Nagy/Alpl+
Pknox1tm1.1Rygo/Pknox1tm1.1Rygo 		involves: 129 * C57BL/6 * C57BL/6JJcl MGI:6887746


Genotype
MGI:6887744
cn1
Allelic
Composition		 Gt(ROSA)26Sortm1(cre/ERT2)Thl/Gt(ROSA)26Sor+
Pknox1tm1.1Rygo/Pknox1tm1.1Rygo
Genetic
Background		 involves: 129 * C57BL/6 * C57BL/6JJcl
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1(cre/ERT2)Thl mutation (0 available); any Gt(ROSA)26Sor mutation (993 available)
Pknox1tm1.1Rygo mutation (1 available); any Pknox1 mutation (90 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
reproductive system phenotype ( J:258387 )
N
• three weeks after tamoxifen treatment, adult testis size and weight is markedly but not significantly decreased relative to similarly treated controls
abnormal spermatogonia morphology ( J:258387 )
• after tamoxifen treatment, GFRalpha1+ cells (corresponding to Asingle~paired spermatogonia in control testis) form Aaligned-like morphologies in adult testes
• c-Kit+ cells aligned more than 32 appear to be absent whereas Aaligned and A1-type spermatogonia are present in control testes, suggesting that c-Kit+ Aaligned spermatogonia fail to differentiate into A1 spermatogonia
decreased male germ cell number ( J:258387 )
• after tamoxifen treatment, most seminiferous tubules lack SCP3+ meiotic cells
azoospermia ( J:258387 )
• epididymides of tamoxifen-treated adult males are devoid of sperm
increased testis apoptosis ( J:258387 )
• after tamoxifen treatment, adult testes show a marked increase in TUNEL+ apoptotic cells relative to similarly treated controls
seminiferous tubule degeneration ( J:258387 )
• after tamoxifen treatment, adult seminiferous tubules appear atrophied and contain very few spermatocytes; however, germ cells beneath the basement membrane (likely spermatogonia and haploid cells) appear to exist
• most seminiferous tubules lack SCP3+ meiotic cells
arrest of spermatogenesis ( J:258387 )
• after tamoxifen treatment, adult spermatogenesis is arrested at the c-Kit+ spermatogonia stage

cellular
abnormal spermatogonia morphology ( J:258387 )
• after tamoxifen treatment, GFRalpha1+ cells (corresponding to Asingle~paired spermatogonia in control testis) form Aaligned-like morphologies in adult testes
• c-Kit+ cells aligned more than 32 appear to be absent whereas Aaligned and A1-type spermatogonia are present in control testes, suggesting that c-Kit+ Aaligned spermatogonia fail to differentiate into A1 spermatogonia
decreased male germ cell number ( J:258387 )
• after tamoxifen treatment, most seminiferous tubules lack SCP3+ meiotic cells
azoospermia ( J:258387 )
• epididymides of tamoxifen-treated adult males are devoid of sperm
increased testis apoptosis ( J:258387 )
• after tamoxifen treatment, adult testes show a marked increase in TUNEL+ apoptotic cells relative to similarly treated controls

endocrine/exocrine glands
increased testis apoptosis ( J:258387 )
• after tamoxifen treatment, adult testes show a marked increase in TUNEL+ apoptotic cells relative to similarly treated controls
seminiferous tubule degeneration ( J:258387 )
• after tamoxifen treatment, adult seminiferous tubules appear atrophied and contain very few spermatocytes; however, germ cells beneath the basement membrane (likely spermatogonia and haploid cells) appear to exist
• most seminiferous tubules lack SCP3+ meiotic cells




Genotype
MGI:6887746
cn2
Allelic
Composition		 Alpltm1(cre)Nagy/Alpl+
Pknox1tm1.1Rygo/Pknox1tm1.1Rygo
Genetic
Background		 involves: 129 * C57BL/6 * C57BL/6JJcl
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Alpltm1(cre)Nagy mutation (6 available); any Alpl mutation (352 available)
Pknox1tm1.1Rygo mutation (1 available); any Pknox1 mutation (90 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
abnormal spermatogonia morphology ( J:258387 )
• GFRalpha1+ cells (corresponding to Asingle~paired spermatogonia in control testis) form Aaligned-like morphologies in adult testes
• adult testes contain relatively few c-Kit+ cells with a weak level of c-Kit expression relative to control testes
• c-Kit+ cells aligned more than 32 appear to be absent whereas Aaligned and A1-type spermatogonia are present in control testes, suggesting that c-Kit+ Aaligned spermatogonia fail to differentiate into A1 spermatogonia
decreased male germ cell number ( J:258387 )
• adult seminiferous tubules lack SCP3+ meiotic cells
azoospermia ( J:258387 )
• adult epididymides are devoid of sperm
increased male germ cell apoptosis ( J:258387 )
• a few seminiferous tubules show accumulation of TUNEL+ apoptotic cells inside the lumen
abnormal spermatogonia proliferation ( J:258387 )
• c-Kit+ spermatogonia appear to be PCNA-negative whereas control c-Kit+ spermatogonia express a high level of PCNA, suggesting a possible proliferation defect
• however, GFRalpha1+ cells (most immature spermatogonial cells) are PCNA-negative, similar to control spermatogonia
seminiferous tubule degeneration ( J:258387 )
• at 3 months of age, nearly all seminiferous tubules appear atrophied and show loss of germ cells
• a few tubules exhibit accumulation of TUNEL+ apoptotic cells inside the lumen
small testis ( J:258387 )
• at 3 months of age, testis size is significantly lower than that in controls
decreased testis weight ( J:258387 )
• at 3 months of age, testis weight is significantly lower than that in controls
arrest of spermatogenesis ( J:258387 )
• adult spermatogenesis is arrested at the c-Kit+ spermatogonia stage

cellular
abnormal spermatogonia morphology ( J:258387 )
• GFRalpha1+ cells (corresponding to Asingle~paired spermatogonia in control testis) form Aaligned-like morphologies in adult testes
• adult testes contain relatively few c-Kit+ cells with a weak level of c-Kit expression relative to control testes
• c-Kit+ cells aligned more than 32 appear to be absent whereas Aaligned and A1-type spermatogonia are present in control testes, suggesting that c-Kit+ Aaligned spermatogonia fail to differentiate into A1 spermatogonia
decreased male germ cell number ( J:258387 )
• adult seminiferous tubules lack SCP3+ meiotic cells
azoospermia ( J:258387 )
• adult epididymides are devoid of sperm
increased male germ cell apoptosis ( J:258387 )
• a few seminiferous tubules show accumulation of TUNEL+ apoptotic cells inside the lumen
abnormal spermatogonia proliferation ( J:258387 )
• c-Kit+ spermatogonia appear to be PCNA-negative whereas control c-Kit+ spermatogonia express a high level of PCNA, suggesting a possible proliferation defect
• however, GFRalpha1+ cells (most immature spermatogonial cells) are PCNA-negative, similar to control spermatogonia

endocrine/exocrine glands
seminiferous tubule degeneration ( J:258387 )
• at 3 months of age, nearly all seminiferous tubules appear atrophied and show loss of germ cells
• a few tubules exhibit accumulation of TUNEL+ apoptotic cells inside the lumen
small testis ( J:258387 )
• at 3 months of age, testis size is significantly lower than that in controls
decreased testis weight ( J:258387 )
• at 3 months of age, testis weight is significantly lower than that in controls




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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory