About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Pknox1tm1.1Rygo
targeted mutation 1.1, Ryo Goitsuka
MGI:6116700
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Gt(ROSA)26Sortm1(cre/ERT2)Thl/Gt(ROSA)26Sor+
Pknox1tm1.1Rygo/Pknox1tm1.1Rygo 		involves: 129 * C57BL/6 * C57BL/6JJcl MGI:6887744
cn2
 		Alpltm1(cre)Nagy/Alpl+
Pknox1tm1.1Rygo/Pknox1tm1.1Rygo 		involves: 129 * C57BL/6 * C57BL/6JJcl MGI:6887746


Genotype
MGI:6887744
cn1
Allelic
Composition		 Gt(ROSA)26Sortm1(cre/ERT2)Thl/Gt(ROSA)26Sor+
Pknox1tm1.1Rygo/Pknox1tm1.1Rygo
Genetic
Background		 involves: 129 * C57BL/6 * C57BL/6JJcl
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1(cre/ERT2)Thl mutation (0 available); any Gt(ROSA)26Sor mutation (997 available)
Pknox1tm1.1Rygo mutation (1 available); any Pknox1 mutation (90 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
reproductive system phenotype ( J:258387 )
N
• three weeks after tamoxifen treatment, adult testis size and weight is markedly but not significantly decreased relative to similarly treated controls
abnormal spermatogonia morphology ( J:258387 )
• after tamoxifen treatment, GFRalpha1+ cells (corresponding to Asingle~paired spermatogonia in control testis) form Aaligned-like morphologies in adult testes
• c-Kit+ cells aligned more than 32 appear to be absent whereas Aaligned and A1-type spermatogonia are present in control testes, suggesting that c-Kit+ Aaligned spermatogonia fail to differentiate into A1 spermatogonia
decreased male germ cell number ( J:258387 )
• after tamoxifen treatment, most seminiferous tubules lack SCP3+ meiotic cells
azoospermia ( J:258387 )
• epididymides of tamoxifen-treated adult males are devoid of sperm
increased testis apoptosis ( J:258387 )
• after tamoxifen treatment, adult testes show a marked increase in TUNEL+ apoptotic cells relative to similarly treated controls
seminiferous tubule degeneration ( J:258387 )
• after tamoxifen treatment, adult seminiferous tubules appear atrophied and contain very few spermatocytes; however, germ cells beneath the basement membrane (likely spermatogonia and haploid cells) appear to exist
• most seminiferous tubules lack SCP3+ meiotic cells
arrest of spermatogenesis ( J:258387 )
• after tamoxifen treatment, adult spermatogenesis is arrested at the c-Kit+ spermatogonia stage

cellular
abnormal spermatogonia morphology ( J:258387 )
• after tamoxifen treatment, GFRalpha1+ cells (corresponding to Asingle~paired spermatogonia in control testis) form Aaligned-like morphologies in adult testes
• c-Kit+ cells aligned more than 32 appear to be absent whereas Aaligned and A1-type spermatogonia are present in control testes, suggesting that c-Kit+ Aaligned spermatogonia fail to differentiate into A1 spermatogonia
decreased male germ cell number ( J:258387 )
• after tamoxifen treatment, most seminiferous tubules lack SCP3+ meiotic cells
azoospermia ( J:258387 )
• epididymides of tamoxifen-treated adult males are devoid of sperm
increased testis apoptosis ( J:258387 )
• after tamoxifen treatment, adult testes show a marked increase in TUNEL+ apoptotic cells relative to similarly treated controls

endocrine/exocrine glands
increased testis apoptosis ( J:258387 )
• after tamoxifen treatment, adult testes show a marked increase in TUNEL+ apoptotic cells relative to similarly treated controls
seminiferous tubule degeneration ( J:258387 )
• after tamoxifen treatment, adult seminiferous tubules appear atrophied and contain very few spermatocytes; however, germ cells beneath the basement membrane (likely spermatogonia and haploid cells) appear to exist
• most seminiferous tubules lack SCP3+ meiotic cells




Genotype
MGI:6887746
cn2
Allelic
Composition		 Alpltm1(cre)Nagy/Alpl+
Pknox1tm1.1Rygo/Pknox1tm1.1Rygo
Genetic
Background		 involves: 129 * C57BL/6 * C57BL/6JJcl
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Alpltm1(cre)Nagy mutation (6 available); any Alpl mutation (352 available)
Pknox1tm1.1Rygo mutation (1 available); any Pknox1 mutation (90 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
abnormal spermatogonia morphology ( J:258387 )
• GFRalpha1+ cells (corresponding to Asingle~paired spermatogonia in control testis) form Aaligned-like morphologies in adult testes
• adult testes contain relatively few c-Kit+ cells with a weak level of c-Kit expression relative to control testes
• c-Kit+ cells aligned more than 32 appear to be absent whereas Aaligned and A1-type spermatogonia are present in control testes, suggesting that c-Kit+ Aaligned spermatogonia fail to differentiate into A1 spermatogonia
decreased male germ cell number ( J:258387 )
• adult seminiferous tubules lack SCP3+ meiotic cells
azoospermia ( J:258387 )
• adult epididymides are devoid of sperm
increased male germ cell apoptosis ( J:258387 )
• a few seminiferous tubules show accumulation of TUNEL+ apoptotic cells inside the lumen
abnormal spermatogonia proliferation ( J:258387 )
• c-Kit+ spermatogonia appear to be PCNA-negative whereas control c-Kit+ spermatogonia express a high level of PCNA, suggesting a possible proliferation defect
• however, GFRalpha1+ cells (most immature spermatogonial cells) are PCNA-negative, similar to control spermatogonia
seminiferous tubule degeneration ( J:258387 )
• at 3 months of age, nearly all seminiferous tubules appear atrophied and show loss of germ cells
• a few tubules exhibit accumulation of TUNEL+ apoptotic cells inside the lumen
small testis ( J:258387 )
• at 3 months of age, testis size is significantly lower than that in controls
decreased testis weight ( J:258387 )
• at 3 months of age, testis weight is significantly lower than that in controls
arrest of spermatogenesis ( J:258387 )
• adult spermatogenesis is arrested at the c-Kit+ spermatogonia stage

cellular
abnormal spermatogonia morphology ( J:258387 )
• GFRalpha1+ cells (corresponding to Asingle~paired spermatogonia in control testis) form Aaligned-like morphologies in adult testes
• adult testes contain relatively few c-Kit+ cells with a weak level of c-Kit expression relative to control testes
• c-Kit+ cells aligned more than 32 appear to be absent whereas Aaligned and A1-type spermatogonia are present in control testes, suggesting that c-Kit+ Aaligned spermatogonia fail to differentiate into A1 spermatogonia
decreased male germ cell number ( J:258387 )
• adult seminiferous tubules lack SCP3+ meiotic cells
azoospermia ( J:258387 )
• adult epididymides are devoid of sperm
increased male germ cell apoptosis ( J:258387 )
• a few seminiferous tubules show accumulation of TUNEL+ apoptotic cells inside the lumen
abnormal spermatogonia proliferation ( J:258387 )
• c-Kit+ spermatogonia appear to be PCNA-negative whereas control c-Kit+ spermatogonia express a high level of PCNA, suggesting a possible proliferation defect
• however, GFRalpha1+ cells (most immature spermatogonial cells) are PCNA-negative, similar to control spermatogonia

endocrine/exocrine glands
seminiferous tubule degeneration ( J:258387 )
• at 3 months of age, nearly all seminiferous tubules appear atrophied and show loss of germ cells
• a few tubules exhibit accumulation of TUNEL+ apoptotic cells inside the lumen
small testis ( J:258387 )
• at 3 months of age, testis size is significantly lower than that in controls
decreased testis weight ( J:258387 )
• at 3 months of age, testis weight is significantly lower than that in controls




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
09/24/2024
MGI 6.24 		The Jackson Laboratory