Phenotypes associated with this allele

• Allele Symbol: Fmnl1^tm1.2Sdb
• Allele Name: targeted mutation 1.2, Scott D Blystone
• Allele ID: MGI:6119535
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Fmnl1^tm1.2Sdb/Fmnl1^tm1.2Sdb Lyz2^tm1(cre)Ifo/Lyz2^tm1(cre)Ifo	involves: C3H * C57BL/6 * FVB/N	MGI:6119817

Genotype
MGI:6119817

cn1

• Allelic Composition: Fmnl1^tm1.2Sdb/Fmnl1^tm1.2Sdb; Lyz2^tm1(cre)Ifo/Lyz2^tm1(cre)Ifo
• Genetic Background: involves: C3H * C57BL/6 * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

hematopoietic system phenotype ( J:250144 )

N • random motility (velocity and distance traveled) of macrophages plated on glass-bottomed dishes in presence of serum for 6 h

N • macrophage phagocytosis for non-specific, CR3-mediated or Fc-receptor-mediated routes of entry

N • adhesion in adherent macrophage cultures

abnormal macrophage morphology ( J:250144 )

• 47.8% decrease in number of macrophages forming podosomes

• 12.6% increase in cell surface area

abnormal Kupffer cell morphology ( J:250144 )

• 38.6% reduction in F4/80 (Adgre1)-positive residential macrophages in liver

decreased macrophage cell number ( J:250144 )

• 38.6% reduction in F4/80 (Adgre1)-positive residential macrophages (Kupffer cells) in liver

• 20% reduction in F4/80 (Adgre1)-positive macrophages in peritoneal cavity after thioglycollate-induced peritonitis

abnormal macrophage physiology ( J:250144 )

• increased amount of activated Rac1 (34.5%), Cdc42 (42.0%) and RhoA (59.4%) protein

impaired macrophage chemotaxis ( J:250144 )

• 50% reduction in migration in Transwell migration assay

immune system

abnormal macrophage morphology ( J:250144 )

• 47.8% decrease in number of macrophages forming podosomes

• 12.6% increase in cell surface area

abnormal Kupffer cell morphology ( J:250144 )

• 38.6% reduction in F4/80 (Adgre1)-positive residential macrophages in liver

decreased macrophage cell number ( J:250144 )

• 38.6% reduction in F4/80 (Adgre1)-positive residential macrophages (Kupffer cells) in liver

• 20% reduction in F4/80 (Adgre1)-positive macrophages in peritoneal cavity after thioglycollate-induced peritonitis

abnormal macrophage physiology ( J:250144 )

• increased amount of activated Rac1 (34.5%), Cdc42 (42.0%) and RhoA (59.4%) protein

impaired macrophage chemotaxis ( J:250144 )

• 50% reduction in migration in Transwell migration assay

cellular

impaired macrophage chemotaxis ( J:250144 )

• 50% reduction in migration in Transwell migration assay

cardiovascular system

cardiovascular system phenotype ( J:250144 )

N • peripheral blood monocyte count

abnormal Kupffer cell morphology ( J:250144 )

• 38.6% reduction in F4/80 (Adgre1)-positive residential macrophages in liver

growth/size/body

growth/size/body region phenotype ( J:250144 )

N • body weight, morphology

N • tissue morphology of lung, liver, kidney, bone, skin, small intestine and large intestine

liver/biliary system

abnormal Kupffer cell morphology ( J:250144 )

• 38.6% reduction in F4/80 (Adgre1)-positive residential macrophages in liver

mortality/aging

mortality/aging ( J:250144 )

N • viable and survive to age 2 years without health abnormalities

reproductive system

reproductive system phenotype ( J:250144 )

N • fecund