Phenotypes associated with this allele

• Allele Symbol: Ptma^tm1.1Got
• Allele Name: targeted mutation 1.1, Otsuka GEN Research Institute
• Allele ID: MGI:6121068
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ptma^tm1.1Got/Ptma^tm1.1Got	involves: C57BL/6J * C57BL/6NTac	MGI:6273614
ht2	Ptma^tm1.1Got/Ptma^+	involves: C57BL/6J * C57BL/6NTac	MGI:6273615

Genotype
MGI:6273614

hm1

• Allelic Composition: Ptma^tm1.1Got/Ptma^tm1.1Got
• Genetic Background: involves: C57BL/6J * C57BL/6NTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality, complete penetrance ( J:240549 )

• no live homozygotes are obtained by heterozygous matings; some embryo-like clusters thought to result from homozygous embryos are found at ~E3.5-E7.5, suggesting early embryonic lethality

Genotype
MGI:6273615

ht2

• Allelic Composition: Ptma^tm1.1Got/Ptma⁺
• Genetic Background: involves: C57BL/6J * C57BL/6NTac

behavior/neurological

behavior/neurological phenotype ( J:240549 )

N • mice show no obsessive behavior in the marble burying test or depression-like behaviors in the forced swim and the tail suspension tests

abnormal learning/memory/conditioning ( J:240549 )

• mice show impaired learning and memory in the step-through passive avoidance test and the KUROBOX test

abnormal passive avoidance behavior ( J:240549 )

• in the step-through passive avoidance test, mice show a significant increase in latency to enter into the dark chamber throughout the conditioning session relative to wild-type controls, suggesting increased freezing behavior upon exposure to the novel environment

• at 24 hr after conditioning, the step-through latency is significantly lower than that in wild-type controls in the retention trials, suggesting impaired learning and memory

impaired cued conditioning behavior ( J:240549 )

• mice exhibit increased freezing behavior in the cued fear conditioning test without exposure to cued (tone) stimulus, but show no significant differences in tone-dependent freezing relative to wild-type controls

impaired spatial learning ( J:240549 )

• mice show impaired spatial discrimination and poor exploratory behavior for foods in the KUROBOX test

increased anxiety-related response ( J:240549 )

• mice exhibit increased anxiety-like behaviors in the light/dark transition test and the novelty-induced hypophagia test

• however, the anxiogenic response is normal in the elevated plus-maze test

decreased locomotor activity ( J:240549 )

• in the open field test, mice show a significant reduction in total distance travelled, time spent in the center area, vertical activity, and stereotypic counts relative to wild-type controls

nervous system

abnormal neuron differentiation ( J:240549 )

• mice show impaired adult neurogenesis in the dentate gyrus of the hippocampus

• in the subgranular zone (SGZ) of the dentate gyrus, the numbers of BrdU-positive cells and doublecortin-positive cells are significantly lower than those in wild-type controls, suggesting decreased neuronal cell proliferation

• however, total brain weight is normal

abnormal neuron proliferation ( J:240549 )

• in the subgranular zone (SGZ) of the dentate gyrus, the numbers of BrdU-positive cells and doublecortin-positive cells are significantly lower than those in wild-type controls, suggesting decreased neuronal cell proliferation

abnormal hippocampus neuron morphology ( J:240549 )

• mice show impaired adult neurogenesis in the dentate gyrus of the hippocampus

abnormal excitatory postsynaptic potential ( J:240549 )

• at 30 min after theta burst stimulation, enhancement of field excitatory post-synaptic potential (fEPSP) is significantly declined in acute hippocampal slices, indicating that LTP induction is weaker than in wild-type controls

reduced long-term potentiation ( J:240549 )

• mice exhibit impaired long-term potentiation induction in the CA1 subfield of hippocampal slices

growth/size/body

decreased body weight ( J:240549 )

• mice exhibit a significant decrease in body weight relative to wild-type controls

homeostasis/metabolism

decreased body temperature ( J:240549 )

• mice exhibit a significant decrease in body temperature relative to wild-type controls

cellular

abnormal neuron differentiation ( J:240549 )

• mice show impaired adult neurogenesis in the dentate gyrus of the hippocampus

• in the subgranular zone (SGZ) of the dentate gyrus, the numbers of BrdU-positive cells and doublecortin-positive cells are significantly lower than those in wild-type controls, suggesting decreased neuronal cell proliferation

• however, total brain weight is normal

abnormal neuron proliferation ( J:240549 )

• in the subgranular zone (SGZ) of the dentate gyrus, the numbers of BrdU-positive cells and doublecortin-positive cells are significantly lower than those in wild-type controls, suggesting decreased neuronal cell proliferation