Analysis Tools|
Allele Symbol Allele Name Allele ID |
Calrtm1.1Osb targeted mutation 1.1, Research Institute for Microbial Diseases, Osaka University MGI:6144082 |
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| Summary |
3 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• in vitro, mouse embryonic fibroblasts (MEFs) isolated from E13.5-E14.5 embryos stop secreting GDF9 and BMP15 into the extracellular fluid within 1-2 days; no mature GDF9 and BMP15 proteins are detected in MEF cells
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
 N |
• when mated with wild-type females, males exhibit normal fertility with no significant differences in the average litter size relative to controls
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
 |
• at 46-48 hours after PMSG injection, oocytes exhibit slightly larger diameters than those collected from control females; however, oocyte number, germinal vesicle breakdown, and in vitro maturation to the MII stage are normal
• oocytes express GDF9 proprotein but lack expression of the 17.5 kDa mature GDF9 protein; however, other fertilization related N-glycoproteins (ZP3 and CD9) are expressed normally
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• in vitro, follicles isolated from 12-day-old mice and cultured for 9 days show defective granulosa-oocyte complex (GOC) development with no detectable increase in average follicular volume
• addition of recombinant GDF9 or BMP15 partially restores granulosa cell proliferation and increases follicular volume
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• corpora lutea are rarely detected after PMSG/hCG treatment
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• at 48 hours after PMSG injection, the ovary surface is smooth and follicles appear abnormal
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• after PMSG/hCG treatment, a few preovulatory follicles are found but most of the follicles at the surface of the ovary are immature
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• after PMSG/hCG treatment, the number of antral follicles/section is significantly greater than in control females
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• after PMSG/hCG treatment, the number of cumulus cells that surround an oocyte after germinal vesicle breakdown is significantly decreased and cumulus expansion is impaired
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• after PMSG/hCG treatment, follicular development as arrested at the early antral stage
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• after PMSG/hCG treatment, ovarian weight is significantly lower than in control females, possibly due to defective folliculogenesis
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• after PMSG/hCG treatment, both the total number of ovulated eggs/mouse and number of metaphase II (MII) eggs/mouse are severely reduced
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• when mated with wild-type males, females show a markedly lower pregnancy rate than control females; only 2 litters with 1 pup each are obtained from 16 copulations with 4 females
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• average litter size is 0.1 +/- 0.3 versus 8.4 +/- 2.6 pups in control females
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• after PMSG/hCG treatment, the fertilization rate (number of 2 cell embryos/number of inseminated eggs) is 0% relative to ~70% in control females
• in vitro fertilization efficiency is lower than in controls; however, MII eggs can be fertilized and develop to term after transfer into pseudopregnant females
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• at 46-48 hours after PMSG injection, oocytes exhibit slightly larger diameters than those collected from control females; however, oocyte number, germinal vesicle breakdown, and in vitro maturation to the MII stage are normal
• oocytes express GDF9 proprotein but lack expression of the 17.5 kDa mature GDF9 protein; however, other fertilization related N-glycoproteins (ZP3 and CD9) are expressed normally
|
|
|
• in vitro, follicles isolated from 12-day-old mice and cultured for 9 days show defective granulosa-oocyte complex (GOC) development with no detectable increase in average follicular volume
• addition of recombinant GDF9 or BMP15 partially restores granulosa cell proliferation and increases follicular volume
|
|
|
• in vitro, follicles isolated from 12-day-old mice and cultured for 9 days show defective granulosa-oocyte complex (GOC) development with no detectable increase in average follicular volume
• addition of recombinant GDF9 or BMP15 partially restores granulosa cell proliferation and increases follicular volume
|
|
|
• corpora lutea are rarely detected after PMSG/hCG treatment
|
|
|
• at 48 hours after PMSG injection, the ovary surface is smooth and follicles appear abnormal
|
|
|
• after PMSG/hCG treatment, a few preovulatory follicles are found but most of the follicles at the surface of the ovary are immature
|
|
|
• after PMSG/hCG treatment, the number of antral follicles/section is significantly greater than in control females
|
|
|
• after PMSG/hCG treatment, the number of cumulus cells that surround an oocyte after germinal vesicle breakdown is significantly decreased and cumulus expansion is impaired
|
|
|
• after PMSG/hCG treatment, follicular development as arrested at the early antral stage
|
|
|
• after PMSG/hCG treatment, ovarian weight is significantly lower than in control females, possibly due to defective folliculogenesis
|
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 12/10/2024 MGI 6.24 |
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