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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Calrtm1.2Osb
targeted mutation 1.2, Research Institute for Microbial Diseases, Osaka University
MGI:6144083
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Calrtm1.2Osb/Calrtm1.1Osb
Tg(Stra8-cre)1Osb/0 		involves: 129S2/SvPas MGI:7639696
cn2
 		Calrtm1.2Osb/Calrtm1.1Osb
Tg(Zp3-cre)93Knw/0 		involves: 129S2/SvPas * C57BL/6J MGI:7639697


Genotype
MGI:7639696
cn1
Allelic
Composition		 Calrtm1.2Osb/Calrtm1.1Osb
Tg(Stra8-cre)1Osb/0
Genetic
Background		 involves: 129S2/SvPas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Calrtm1.1Osb mutation (2 available); any Calr mutation (14 available)
Calrtm1.2Osb mutation (0 available); any Calr mutation (14 available)
Tg(Stra8-cre)1Osb mutation (2 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
reproductive system phenotype ( J:250114 )
N
• when mated with wild-type females, males exhibit normal fertility with no significant differences in the average litter size relative to controls




Genotype
MGI:7639697
cn2
Allelic
Composition		 Calrtm1.2Osb/Calrtm1.1Osb
Tg(Zp3-cre)93Knw/0
Genetic
Background		 involves: 129S2/SvPas * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Calrtm1.1Osb mutation (2 available); any Calr mutation (14 available)
Calrtm1.2Osb mutation (0 available); any Calr mutation (14 available)
Tg(Zp3-cre)93Knw mutation (2 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
abnormal oocyte morphology ( J:250114 )
• at 46-48 hours after PMSG injection, oocytes exhibit slightly larger diameters than those collected from control females; however, oocyte number, germinal vesicle breakdown, and in vitro maturation to the MII stage are normal
• oocytes express GDF9 proprotein but lack expression of the 17.5 kDa mature GDF9 protein; however, other fertilization related N-glycoproteins (ZP3 and CD9) are expressed normally
decreased granulosa cell proliferation ( J:250114 )
• in vitro, follicles isolated from 12-day-old mice and cultured for 9 days show defective granulosa-oocyte complex (GOC) development with no detectable increase in average follicular volume
• addition of recombinant GDF9 or BMP15 partially restores granulosa cell proliferation and increases follicular volume
decreased corpora lutea number ( J:250114 )
• corpora lutea are rarely detected after PMSG/hCG treatment
abnormal ovarian follicle morphology ( J:250114 )
• at 48 hours after PMSG injection, the ovary surface is smooth and follicles appear abnormal
decreased mature ovarian follicle number ( J:250114 )
• after PMSG/hCG treatment, a few preovulatory follicles are found but most of the follicles at the surface of the ovary are immature
increased tertiary ovarian follicle number ( J:250114 )
• after PMSG/hCG treatment, the number of antral follicles/section is significantly greater than in control females
decreased cumulus expansion ( J:250114 )
• after PMSG/hCG treatment, the number of cumulus cells that surround an oocyte after germinal vesicle breakdown is significantly decreased and cumulus expansion is impaired
impaired ovarian folliculogenesis ( J:250114 )
• after PMSG/hCG treatment, follicular development as arrested at the early antral stage
decreased ovary weight ( J:250114 )
• after PMSG/hCG treatment, ovarian weight is significantly lower than in control females, possibly due to defective folliculogenesis
decreased superovulation rate ( J:250114 )
• after PMSG/hCG treatment, both the total number of ovulated eggs/mouse and number of metaphase II (MII) eggs/mouse are severely reduced
reduced female fertility ( J:250114 )
• when mated with wild-type males, females show a markedly lower pregnancy rate than control females; only 2 litters with 1 pup each are obtained from 16 copulations with 4 females
decreased litter size ( J:250114 )
• average litter size is 0.1 +/- 0.3 versus 8.4 +/- 2.6 pups in control females
decreased fertilization frequency ( J:250114 )
• after PMSG/hCG treatment, the fertilization rate (number of 2 cell embryos/number of inseminated eggs) is 0% relative to ~70% in control females
• in vitro fertilization efficiency is lower than in controls; however, MII eggs can be fertilized and develop to term after transfer into pseudopregnant females

cellular
abnormal oocyte morphology ( J:250114 )
• at 46-48 hours after PMSG injection, oocytes exhibit slightly larger diameters than those collected from control females; however, oocyte number, germinal vesicle breakdown, and in vitro maturation to the MII stage are normal
• oocytes express GDF9 proprotein but lack expression of the 17.5 kDa mature GDF9 protein; however, other fertilization related N-glycoproteins (ZP3 and CD9) are expressed normally
decreased granulosa cell proliferation ( J:250114 )
• in vitro, follicles isolated from 12-day-old mice and cultured for 9 days show defective granulosa-oocyte complex (GOC) development with no detectable increase in average follicular volume
• addition of recombinant GDF9 or BMP15 partially restores granulosa cell proliferation and increases follicular volume

endocrine/exocrine glands
decreased granulosa cell proliferation ( J:250114 )
• in vitro, follicles isolated from 12-day-old mice and cultured for 9 days show defective granulosa-oocyte complex (GOC) development with no detectable increase in average follicular volume
• addition of recombinant GDF9 or BMP15 partially restores granulosa cell proliferation and increases follicular volume
decreased corpora lutea number ( J:250114 )
• corpora lutea are rarely detected after PMSG/hCG treatment
abnormal ovarian follicle morphology ( J:250114 )
• at 48 hours after PMSG injection, the ovary surface is smooth and follicles appear abnormal
decreased mature ovarian follicle number ( J:250114 )
• after PMSG/hCG treatment, a few preovulatory follicles are found but most of the follicles at the surface of the ovary are immature
increased tertiary ovarian follicle number ( J:250114 )
• after PMSG/hCG treatment, the number of antral follicles/section is significantly greater than in control females
decreased cumulus expansion ( J:250114 )
• after PMSG/hCG treatment, the number of cumulus cells that surround an oocyte after germinal vesicle breakdown is significantly decreased and cumulus expansion is impaired
impaired ovarian folliculogenesis ( J:250114 )
• after PMSG/hCG treatment, follicular development as arrested at the early antral stage
decreased ovary weight ( J:250114 )
• after PMSG/hCG treatment, ovarian weight is significantly lower than in control females, possibly due to defective folliculogenesis




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Send questions and comments to User Support. 		last database update
11/19/2024
MGI 6.24 		The Jackson Laboratory