Phenotypes associated with this allele

• Allele Symbol: Zcchc17^tm2Pou
• Allele Name: targeted mutation 2, Pin Ouyang
• Allele ID: MGI:6147734
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Zcchc17^tm2Pou/Zcchc17^tm2Pou	involves: 129 * C57BL/6J	MGI:6148077

Genotype
MGI:6148077

hm1

• Allelic Composition: Zcchc17^tm2Pou/Zcchc17^tm2Pou
• Genetic Background: involves: 129 * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal cell differentiation ( J:233160 )

• adipogenic differentiation of mesenchymal stem cells (MSCs) in vitro 7 days after induction

• ~50% reduction in osteogenic differentiation of mesenchymal stem cells (MSCs) in vitro 7 days after induction

early cellular replicative senescence ( J:233160 )

• senescence-associated-beta-galatosidase staining in notochord and radius, ulna and digit bones of E12.5 embryo

• senescence-associated-beta-galatosidase staining in mesenchymal stem cells (MSCs)

• no senescence-associated-beta-galatosidase staining in mouse embryo fibroblasts (MEFs)

• 4-fold increase in level of reactive oxygen species in MSCs in vitro according to DCFH-DA labelling

• increased expression of Rb1 and Cdkn2a proteins in MSCs in vitro

oxidative stress ( J:233160 )

• 4-fold increase in level of reactive oxygen species in mesenchymal stem cells (MSCs) in vitro according to DCFH-DA labelling

• increased Cyp1b1 protein expression in MSCs in vitro

embryo

embryo phenotype ( J:233160 )

N • embryos develop normally and mice are born at expected Mendelian ratios

reproductive system

reproductive system phenotype ( J:233160 )

N • fertile