All Phenotypes Ano5<tm1.2Naga> MGI Mouse

reproductive system phenotype ( J:236134 )

N

• sperm count in cauda epididymis and sperm morphology

• testis and epididymis weights and testis morphology

• sperm flagella beating ability

• sperm binding to zona pellucida of cumulus-free eggs

• sperm ATP content and mitochondrial membrane potential

• tyrosine phosphorylation of sperm proteins and phosphatidylserine (PtdSer) exposure on sperm head

decreased activated sperm motility ( J:236134 )

• significantly reduced VAP (average path velocity), VSL (straight-line velocity) and VCL (curvilinear velocity) of cauda epididymis sperm in TYH medium

• ~50% reduction in number of cauda epididymis sperm moving in straight line in TYH medium after capacitation

decreased sperm progressive motility ( J:236134 )

• significantly reduced VSL (straight-line velocity) of cauda epididymis sperm in TYH medium

• reduced number of cauda epididymis sperm moving in straight line after 2 hours in TYH medium (compared to 10 minutes)

reduced male fertility ( J:236134 )

• yield around half of pups (compared to wild-type males) when mated to wild-type females

• fewer than 25% cumulus-intact eggs from super-ovulated B6D2F1 mice were fertilized by capacitated homozygous mutant sperm (vs 90% with heterozygous mutant sperm) in vitro

• failure of sperm to fertilize hyaluronidase-treated cumulus-free eggs in vitro; sperm can fertilize collagenase-treated zona-free eggs

homeostasis/metabolism phenotype ( J:236134 )

N	• serum creatine kinase level

muscle phenotype ( J:236134 )

N	• no muscle phenotype • forced-running test performance

behavior/neurological phenotype ( J:236134 )

N	• forced-running test performance