Phenotypes associated with this allele

• Allele Symbol: Pgp^tm1.2Ango
• Allele Name: targeted mutation 1.2, Antje Gohla
• Allele ID: MGI:6150833
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pgp^tm1.2Ango/Pgp^tm1.2Ango	involves: 129S4/SvJaeSor * C57BL/6J * FVB/N	MGI:6150904

Genotype
MGI:6150904

hm1

• Allelic Composition: Pgp^tm1.2Ango/Pgp^tm1.2Ango
• Genetic Background: involves: 129S4/SvJaeSor * C57BL/6J * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal cell physiology ( J:259970 )

• at normoxic (20% O2) conditions in E8.5 embryo explant lysates

• 34% reduced triosephosphate isomerase activity

• increased glycerol-3-phosphate levels

• triosephosphate isomerase activity under hypoxic (1% O2) conditions

• increased diacylglycerol (DG 32:0, 34:0 and 36:0) levels

• increased triglyceride (TG) levels

• reduced phosphatidylcholine levels

• 13% increase in DG/TG ratio

• 2.7-fold increase in TG/phosphatidylcholine ratio

• increased presence and abundance of perilipin-3-positive lipid droplets in MEFs: in 26.4% of cells with 47.8 droplets/cell versus 22% with 35.8 droplets/cell for wild-type

• more than 2-fold increase in Dgat2 expression, and increase in Cebpa and Cebpb expression according to qRT-PCR

• triglyceride (TG) and phosphatidylcholine levels in MEFs isolated from embryos under hypoxic (1% O2) conditions

abnormal fibroblast proliferation ( J:259970 )

• mouse embryo fibroblasts (MEFs) isolated from E8.5 embryos by dissociation fail to grow under normoxic (20% O2) conditions

• growth of undissociated explanted E8.5 embryos under normoxic (20% O2) conditions for 7 days: beating hearts and cellular outgrowths

• proliferation under hypoxic conditions (1% O2) of MEFs isolated from E8.5 embryos by dissociation

embryo

delayed embryo turning ( J:259970 )

• at E9.5 none of 8 embryos had completed turning, compared to 8 of 10 wild-type and 22 of 24 heterozygotes that had

embryonic growth retardation ( J:259970 )

• at E9.5, embryos were size of E8.5 wild-type embryos

• at E10.5, 80% of embryos showed retarded growth, being size of E9.5 wild-type embryos

decreased embryo size ( J:259970 )

• at E9.5, embryos were size of E8.5 wild-type embryos

• at E10.5, 80% of embryos showed retarded growth, being size of E9.5 wild-type embryos

abnormal somite development ( J:259970 )

• stalled development after E8.5

• somite pair numbers at E8.5

abnormal placenta morphology ( J:259970 )

• impaired vessel formation in labyrinth, indicated by strong reduction of Flk1- and Sm22-positive structures

• outer layer of Pl1- or Hand1-positive trophoblast giant cells

• middle layer of Tbpb- or Flt1-positive spongiotrophoblast cells

abnormal placenta labyrinth morphology ( J:259970 )

• impaired vessel formation, indicated by strong reduction of Flk1- and Sm22-positive structures

• decrease in embryonic blood vessel density: 13/87% ratio of embryonic/maternal vessels, versus 44/56% in wild-type

small placenta ( J:259970 )

cardiovascular system

abnormal heart morphology ( J:259970 )

• very few embryos with beating heart at E10.5

hemorrhage ( J:259970 )

• cranial, dorsal and abdominal bleeding in some embryos at E10.5, increasing at E11.5

growth/size/body

embryonic growth retardation ( J:259970 )

• at E9.5, embryos were size of E8.5 wild-type embryos

• at E10.5, 80% of embryos showed retarded growth, being size of E9.5 wild-type embryos

decreased embryo size ( J:259970 )

• at E9.5, embryos were size of E8.5 wild-type embryos

• at E10.5, 80% of embryos showed retarded growth, being size of E9.5 wild-type embryos

mortality/aging

embryonic lethality, complete penetrance ( J:259970 )

• normal Mendelian ratio between E8.5 and E11.5 but only 1 homozygous mutant at E12.5 out of 25 embryos tested at that stage after heterozygote matings

• no mice found at age P21