Phenotypes associated with this allele

• Allele Symbol: Slfn8^tm1Aki
• Allele Name: targeted mutation 1, Shizuo Akira
• Allele ID: MGI:6151479
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Slfn8^tm1Aki/Slfn8^tm1Aki	involves: 129X1/SvJ * C57BL/6	MGI:6690806

Genotype
MGI:6690806

hm1

• Allelic Composition: Slfn8^tm1Aki/Slfn8^tm1Aki
• Genetic Background: involves: 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:258797 )

N • mice exhibit normal immune cell populations, including macrophages, neutrophils, T cells and B cells, relative to wild-type controls

N • in vitro, CD4+ T cells show normal proliferation in response to anti-CD3/anti-CD28 stimulation and peritoneal macrophages show normal levels of TNF alpha production in response to TLR ligands (including Pam3CSK4, LPS and R848)

decreased interferon-gamma secretion ( J:258797 )

• draining lymph node cells and splenocytes from MOG_35-55 immunized mice produce significantly less IFN-gamma in response to MOG_35-55 peptide in vitro

• after MOG_35-55 immunization, production of IFN-gamma by irradiated recipient mice transplanted with wild-type bone marrow is significantly reduced relative to irradiated wild-type recipient mice transplanted with wild-type bone marrow

decreased interleukin-1 beta secretion ( J:258797 )

• IL-1beta protein level is reduced in homogenized lymph nodes from MOG_35-55 immunized mice

• IL-1beta protein level in spleen is normal after MOG_35-55 immunization

decreased interleukin-17 secretion ( J:258797 )

• draining lymph node cells and splenocytes from MOG_35-55 immunized mice produce significantly less IL-17A in response to MOG_35-55 peptide in vitro

• after MOG_35-55 immunization, production of IL-17A by irradiated recipient mice transplanted with wild-type bone marrow is significantly reduced relative to irradiated wild-type recipient mice transplanted with wild-type bone marrow

decreased interleukin-6 secretion ( J:258797 )

• IL-6 protein level is reduced in homogenized lymph nodes from MOG_35-55 immunized mice

• Il6 mRNA levels are significantly reduced in CD45- CD31+ endothelial cells from MOG_35-55 immunized mice

• IL-6 protein level in spleen is normal after MOG_35-55 immunization

• peritoneal macrophages show normal IL-6 production in response to TLR ligands (including Pam3CSK4, LPS and R848)

decreased susceptibility to experimental autoimmune encephalomyelitis ( J:258797 )

• mice exhibit lower clinical scores and a reduced number of mononuclear cells in the spinal cord at day 24 after MOG_35-55 peptide-induced experimental autoimmune encephalitis (EAE) relative to wild-type controls