All Phenotypes Zc3h4<em1(IMPC)Mbp> MGI Mouse

cellular phenotype ( J:301719 )

N	• blastocysts exhibit low reactive oxygen species (ROS) levels, similar to wild-type and heterozygous controls, indicating normal oxidative homeostasis

increased embryonic tissue cell apoptosis ( J:301719 )

• blastocysts show a significantly higher percentage of TUNEL-positive nuclei than controls, indicating increased DNA breaks and cell death

decreased cell proliferation ( J:301719 )

• blastocysts show a significantly lower percentage of EdU-positive nuclei than controls, indicating decreased cell proliferation

absent inner cell mass proliferation ( J:279207 , J:301719 )

• blastocysts fail to form typical outgrowth colonies after 3 days in culture (J:279207)

• in vitro outgrowth assays show that 6 of 11 blastocysts hatch but fail to form a typical inner cell mass (ICM) colony after 3 days in culture (J:301719)

failure of blastocyst to hatch from the zona pellucida ( J:279207 , J:301719 )

• blastocysts fail to hatch from the zona pellucida after 3 days in culture (J:279207)

• in vitro outgrowth assays show that 5 of 11 fail to hatch from the zona pellucida with all blastomeres trapped in the zona after 3 days in culture (J:301719)

embryonic growth arrest ( J:301719 )

• embryos fail to develop beyond blastocyst formation

abnormal embryonic epiblast morphology ( J:301719 )

• both the number of NANOG-positive cells per blastocyst and percentage of NANOG-positive epiblast cells is significantly reduced, indicating a defective epiblast lineage with far fewer cells

abnormal primitive endoderm morphology ( J:301719 )

• both the number of SOX17-positive cells per blastocyst and percentage of SOX17-positive primitive endoderm cells is significantly reduced, indicating a defective primitive endoderm lineage with far fewer cells

abnormal blastocyst morphology ( J:301719 )

• total cell number per blastocyst is significantly lower than in controls

• however, no active TRP53-positive cells are detected, similar to controls

abnormal inner cell mass morphology ( J:301719 )

• number of POU5F1/OCT4-positive cells per blastocyst is severely decreased; percentage of POU5F1/OCT4-positive ICM cells is significantly reduced, indicating far fewer ICM specified cells

abnormal trophectoderm morphology ( J:301719 )

• number of CDX2-positive cells per blastocyst is severely reduced but the percentage of CDX2-positive cells is significantly increased, indicating more trophectoderm (TE) specified cells

abnormal embryonic tissue physiology ( J:301719 )

• blastocysts show a significantly lower percentage of EdU-positive nuclei than controls, indicating decreased cell proliferation

increased embryonic tissue cell apoptosis ( J:301719 )

• blastocysts show a significantly higher percentage of TUNEL-positive nuclei than controls, indicating increased DNA breaks and cell death

absent inner cell mass proliferation ( J:279207 , J:301719 )

• blastocysts fail to form typical outgrowth colonies after 3 days in culture (J:279207)

• in vitro outgrowth assays show that 6 of 11 blastocysts hatch but fail to form a typical inner cell mass (ICM) colony after 3 days in culture (J:301719)

prenatal lethality prior to heart atrial septation ( J:211773 )

IMPC - UCD

embryonic lethality prior to organogenesis ( J:211773 )

IMPC - UCD

embryonic lethality prior to organogenesis, complete penetrance ( J:279207 )

• normal looking blastocysts are present in expected Mendelian ratios at E3.5; excess resorption sites but no homozygous embryos are observed at E7.5

embryonic lethality at implantation, complete penetrance ( J:301719 )

• embryos exhibit peri-implantation lethality; an increased number of empty decidua is noted at E7.5, suggesting that most embryos elicit a decidual response but likely degenerate and resorb with no embryos found at later stages

preweaning lethality, complete penetrance ( J:211773 )

IMPC - UCD

empty decidua capsularis ( J:301719 )

• an increased number of empty decidua is noted at E7.5

failure of embryo implantation ( J:301719 )

• embryos fail to implant in the uterus

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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