Analysis Tools
cellular
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• altered gene expression in 224 loci due to changes in frequency of 5-methylcytosine (5mC) and its oxidized derivatives 5hmC, 5fC and 5caC in embryonic stem cells
• altered gene expression due to differentially methylated regions (hyper- and hypomethylated relative to wild-type) in E9.5 embryos
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• increase in 5-methylcytosine (5mC) and Tet-oxidized 5mC derivatives 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxycytosine (5caC) levels in embryonic stem cells
• increase in 5mC and 5hmC levels in E9.5 embryos
• increased fraction of highly methylated CpG-enriched regions in E9.5 embryos
• 1589 differentially methylated regions (hyper- and hypomethylated relative to wild-type) in E9.5 embryos
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growth/size/body
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• underweight at age 3 weeks
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mortality/aging
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• significantly fewer embryos found than expected Mendelian ratio at E9.5-E16.5
• significantly fewer pups born than expected Mendelian ratio
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• significantly fewer embryos found than expected Mendelian ratio at E9.5-E16.5 but no empty implantation sites or absorbed conceptuses in utero
• significantly fewer pups born than expected Mendelian ratio
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reproductive system
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• from in-cross of homozygous mutants
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