Phenotypes associated with this allele

• Allele Symbol: Becn1^tm2.1Blev
• Allele Name: targeted mutation 2.1, Beth Levine
• Allele ID: MGI:6156896
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Becn1^tm2.1Blev/Becn1^tm2.1Blev	B6.129-Becn1^tm2.1Blev	MGI:6257017
cx2	Becn1^tm2.1Blev/Becn1^tm2.1Blev Tg(APPV717F)109Ili/0	B6.Cg-Becn1^tm2.1Blev Tg(APPV717F)109Ili	MGI:6257023
cx3	Becn1^tm2.1Blev/Becn1^tm2.1Blev Tg(CAG-EGFP/Map1lc3b)53Nmz/0	involves: 129 * C57BL/6 * C57BL/6J * C57BL/6NCrlj * DBA/2	MGI:6257019
cx4	Becn1^tm2.1Blev/Becn1^tm2.1Blev Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/0	involves: 129 * C57BL/6 * C57BL/6J * SJL	MGI:6257020

Genotype
MGI:6257017

hm1

• Allelic Composition: Becn1^tm2.1Blev/Becn1^tm2.1Blev
• Genetic Background: B6.129-Becn1^tm2.1Blev

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:244104 )

• mice are fertile, of normal size and weight, and show normal histology in major organs

Genotype
MGI:6257023

cx2

• Allelic Composition: Becn1^tm2.1Blev/Becn1^tm2.1Blev; Tg(APPV717F)109Ili/0
• Genetic Background: B6.Cg-Becn1^tm2.1Blev Tg(APPV717F)109Ili

mortality/aging

extended life span ( J:244104 )

• mice exhibit decreased mortality compared to single Tg(APPV717F)109Ili expressing mice

Genotype
MGI:6257019

cx3

• Allelic Composition: Becn1^tm2.1Blev/Becn1^tm2.1Blev; Tg(CAG-EGFP/Map1lc3b)53Nmz/0
• Genetic Background: involves: 129 * C57BL/6 * C57BL/6J * C57BL/6NCrlj * DBA/2

cellular

enhanced autophagy ( J:244104 )

• under fed and resting conditions, mice exhibit a higher number of autophagosomes in skeletal muscle and brain, indicating high basal autophagy

homeostasis/metabolism

enhanced autophagy ( J:244104 )

• under fed and resting conditions, mice exhibit a higher number of autophagosomes in skeletal muscle and brain, indicating high basal autophagy

Genotype
MGI:6257020

cx4

• Allelic Composition: Becn1^tm2.1Blev/Becn1^tm2.1Blev; Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/0
• Genetic Background: involves: 129 * C57BL/6 * C57BL/6J * SJL

behavior/neurological

behavior/neurological phenotype ( J:244104 )

N • mice show improved performance day by day in both the escape latency and distance during the hidden platform trails of the Morris water maze compared to single Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas expressing mice

homeostasis/metabolism

amyloid beta deposits ( J:244104 )

• mice show lower levels of both soluble and insoluble amyloid beta 42 in the brain than in single Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas expressing mice

• however, expression of the precursor APP remains unaffected

• mice show a reduction of amyloid plaques in the cortex and a trend of reduction in the hippocampus

• short-term treatment of mice with SBI-0206965, an autophagy inhibitor blocking the kinase activity of an essential upstream kinase ULK1, abolishes the reduction in brain amyloid beta 42 levels

nervous system

amyloid beta deposits ( J:244104 )

• mice show lower levels of both soluble and insoluble amyloid beta 42 in the brain than in single Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas expressing mice

• however, expression of the precursor APP remains unaffected

• mice show a reduction of amyloid plaques in the cortex and a trend of reduction in the hippocampus

• short-term treatment of mice with SBI-0206965, an autophagy inhibitor blocking the kinase activity of an essential upstream kinase ULK1, abolishes the reduction in brain amyloid beta 42 levels