Phenotypes associated with this allele

• Allele Symbol: Tmem59^tm1.1Tqw
• Allele Name: targeted mutation 1.1, Tieqiao Wen
• Allele ID: MGI:6158675
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tmem59^tm1.1Tqw/Tmem59^tm1.1Tqw	involves: 129P2/OlaHsd * C57BL/6	MGI:6158677
cx2	Tmem59^tm1.1Tqw/Tmem59^tm1.1Tqw Tg(Thy1-EGFP)MJrs/0	involves: 129P2/OlaHsd * C57BL/6J * CBA	MGI:6158678

Genotype
MGI:6158677

hm1

• Allelic Composition: Tmem59^tm1.1Tqw/Tmem59^tm1.1Tqw
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal dendrite morphology ( J:261729 )

• hippocampal neurons show reduced dendritic complexity after 9 days in culture

abnormal dendritic spine morphology ( J:261729 )

• spine head are is reduced by 20%

decreased dendritic spine density ( J:261729 )

• 6.3% and 18.4% reduction in density in the CA1 and CA3 areas of the hippocampus

abnormal synapse morphology ( J:261729 )

• 15.9% decrease in synaptic density in the CA3 area of the hippocampus

abnormal postsynaptic density morphology ( J:261729 )

• average length is reduced by 16.1%

abnormal neuron physiology ( J:261729 )

• 32.6% reduction in dendritic spine motility in cultured hippocampal neurons

abnormal miniature excitatory postsynaptic currents ( J:261729 )

• frequency, but not amplitude, is significantly reduced in hippocampal cells

behavior/neurological

abnormal inhibitory learning ( J:261729 )

• impaired performance on the reversal learning task in a Morris water maze

abnormal response to novelty ( J:261729 )

• in an object relocation test mice spend less time exploring a displaced object than a non-displaced object, in controls this is reversed

decreased exploration in new environment ( J:261729 )

• in a Y maze test

Genotype
MGI:6158678

cx2

• Allelic Composition: Tmem59^tm1.1Tqw/Tmem59^tm1.1Tqw; Tg(Thy1-EGFP)MJrs/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J * CBA

nervous system

abnormal dendrite morphology ( J:261729 )

• dendritic arborizations are much simpler in layer II/III of the cerebral cortex at P90

• total dendritic length is decreased by 27.8% and secondary and tertiary branching levels are significantly reduced

decreased dendritic spine density ( J:261729 )

• spine density is reduced by 15.6% on apical dendrites

• spine density in layer V/VI of the cortex is reduced at P42 and P90

• spine density in layer V/VI of the cortex remains relatively constant from P21 to P42 to P90 unlike in wild-type controls where spine density increases across these ages