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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Plek2tm1Pji
targeted mutation 1, Peng Ji
MGI:6162244
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Plek2tm1Pji/Plek2tm1Pji involves: 129S1/Sv * 129X1/SvJ * C57BL/6 MGI:6356743
cn2
 		Plek2tm1Pji/Plek2tm1Pji
Jak2tm1.1Ble/Jak2+
Commd10Tg(Vav1-icre)A2Kio/Commd10+ 		involves: 129 * C57BL/6 * C57BL/10 * CBA/Ca MGI:6356967


Genotype
MGI:6356743
hm1
Allelic
Composition		 Plek2tm1Pji/Plek2tm1Pji
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Plek2tm1Pji mutation (0 available); any Plek2 mutation (17 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal granulocyte differentiation ( J:257910 )
• granulocytic differentiation to Gr1/Mac1 double-positive cells is mildly decreased in cultured cells
abnormal megakaryocyte differentiation ( J:257910 )
• early-stage megakaryocytic differentiation to CD41+ cells is mildly decreased in cultured cells
enlarged spleen ( J:257910 )
• spleen is slightly enlarged
abnormal erythropoiesis ( J:257910 )
• mice develop mild ineffective erythropoiesis, showing slightly increased erythroblasts and decreased reticulocytes in bone marrow
anemia ( J:257910 )
• mice develop minor anemia after 1 year of age
• however, mice do not exhibit hematologic abnormalities at 2-4 months of age
increased erythroblast number ( J:257910 )
• slight increase in erythroblasts in the bone marrow
abnormal reticulocyte cell number ( J:257910 )
• slight decrease in reticulocytes in the bone marrow

immune system
abnormal granulocyte differentiation ( J:257910 )
• granulocytic differentiation to Gr1/Mac1 double-positive cells is mildly decreased in cultured cells
enlarged spleen ( J:257910 )
• spleen is slightly enlarged

cellular
increased cellular sensitivity to oxidative stress ( J:257910 )
• peripheral red blood cells exhibit increased sensitivity to oxidative damage
abnormal cell differentiation ( J:257910 )
• erythroid differentiation to Ter119/CD71 double-positive cells is mildly decreased in cultured cells
abnormal granulocyte differentiation ( J:257910 )
• granulocytic differentiation to Gr1/Mac1 double-positive cells is mildly decreased in cultured cells
abnormal megakaryocyte differentiation ( J:257910 )
• early-stage megakaryocytic differentiation to CD41+ cells is mildly decreased in cultured cells

growth/size/body
enlarged spleen ( J:257910 )
• spleen is slightly enlarged




Genotype
MGI:6356967
cn2
Allelic
Composition		 Plek2tm1Pji/Plek2tm1Pji
Jak2tm1.1Ble/Jak2+
Commd10Tg(Vav1-icre)A2Kio/Commd10+
Genetic
Background		 involves: 129 * C57BL/6 * C57BL/10 * CBA/Ca
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Commd10Tg(Vav1-icre)A2Kio mutation (3 available); any Commd10 mutation (24 available)
Jak2tm1.1Ble mutation (1 available); any Jak2 mutation (59 available)
Plek2tm1Pji mutation (0 available); any Plek2 mutation (17 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
premature death ( J:257910 )
• more than 80% of mice survive beyond 40 weeks
• mice treated with phenylhydrazine to further reduce red blood counts show an even greater improvement in survival

hematopoietic system
hematopoietic system phenotype ( J:257910 )
N
• mice exhibit amelioration of the neutrophilia and thrombocytosis, partial reversion of reticulocytosis, mild reduction in red blood cell counts, fewer megakaryocytes and megakaryocyte clusters, reduced spleen size, and reversion of about 25% of the red blood cell expansion seen in single Jak2tm1.1Ble conditional mutants
• bone marrow lineage-negative hematopoietic stem and progenitor cells grown in culture show a reduction in the increased proliferation of erythroid and granulocytic cells seen in single Jak2tm1.1Ble conditional mutants
abnormal granulocyte differentiation ( J:257910 )
• granulocytic differentiation to Gr1/Mac1 double-positive cells is mildly decreased in cultured cells
abnormal megakaryocyte differentiation ( J:257910 )
• early-stage megakaryocytic differentiation to CD41+ cells is mildly decreased in cultured cells

homeostasis/metabolism
homeostasis/metabolism phenotype ( J:257910 )
N
• mice show decreased vascular occlusions than in single Jak2tm1.1Ble conditional mutants, with a decrease in the number of large clots with compact red blood cells and improved blood flow

immune system
immune system phenotype ( J:257910 )
N
• several proinflammatory cytokines, including CCL3 and CSF1, that are increased in single Jak2tm1.1Ble conditional mutants are reduced
abnormal granulocyte differentiation ( J:257910 )
• granulocytic differentiation to Gr1/Mac1 double-positive cells is mildly decreased in cultured cells

cellular
abnormal cell differentiation ( J:257910 )
• erythroid differentiation to Ter119/CD71 double-positive cells is mildly decreased in cultured cells
abnormal granulocyte differentiation ( J:257910 )
• granulocytic differentiation to Gr1/Mac1 double-positive cells is mildly decreased in cultured cells
abnormal megakaryocyte differentiation ( J:257910 )
• early-stage megakaryocytic differentiation to CD41+ cells is mildly decreased in cultured cells




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Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory