All Phenotypes Trim8<tm1Hbsh> MGI Mouse

increased susceptibility to bacterial infection induced morbidity/mortality ( J:251270 )

• after oral administration of Salmonella typhimurium, mice exhibit earlier death onset and a higher percentage of lethality within 12 days post-infection than wild-type controls

increased susceptibility to induced morbidity/mortality ( J:251270 )

• after i.p. injection of poly(I:C) plus D-galactosamine or LPS, mice exhibit earlier death onset and a higher percentage of lethality within 42 h post-injection relative to wild-type controls

immune system phenotype ( J:251270 )

N	• untreated mice exhibit normal immune cell development with no significant alterations in total cell numbers and compositions of major immune cells in spleens, thymi, and lymph nodes

increased macrophage cytokine production ( J:251270 )

• poly(I:C)- and LPS- but not Sendai virus (SeV)-, PGN- or R848-induced secretion of IFN-beta, TNF, and IL6 is significantly increased in bone marrow-derived macrophages (BMDMs)

abnormal circulating chemokine level ( J:251270 )

• 2 h after i.p. injection of poly(I:C) plus D-galactosamine or LPS, mice exhibit significantly higher serum CCL5 (also known as RANTES) levels than wild-type controls

increased circulating interleukin-6 level ( J:251270 )

• 2 h after i.p. injection of poly(I:C) plus D-galactosamine or LPS, mice exhibit significantly higher serum IL6 levels than wild-type controls

increased circulating tumor necrosis factor level ( J:251270 )

• 2 h after i.p. injection of poly(I:C) plus D-galactosamine or LPS, mice exhibit significantly higher serum TNF levels than wild-type controls

abnormal chemokine secretion ( J:251270 )

• poly(I:C)- and LPS-induced transcription of Ccl5 [chemokine (C-C motif) ligand 5, also known as Rantes] is significantly increased in bone marrow-derived macrophages (BMDMs) and bone marrow-derived dendritic cells (BMDCs)

• poly(I:C)- and LPS- but not Sendai virus (SeV)-induced transcription of Ccl5 is markedly increased in mouse embryonic fibroblasts (MEFs)

• reconstitution of TRIM8 markedly inhibits poly(I:C)- and LPS-triggered transcription of Ccl5 in MEFs

• induction of Ccl5 mRNA by R848, PGN, or Sendai virus is normal in BMDMs and BMDCs

increased interferon-beta secretion ( J:251270 )

• poly(I:C)- and LPS-induced transcription of Ifnb1 is significantly increased in BMDMs and BMDCs

• poly(I:C)- and LPS- but not Sendai virus (SeV)-, PGN- or R848-induced secretion of IFN-beta is significantly increased in BMDMs

• poly(I:C)- and LPS- but not SeV-induced transcription of Ifnb1 is markedly increased in MEFs

• reconstitution of TRIM8 markedly inhibits poly(I:C)- and LPS-triggered transcription of Ifnb1 in MEFs

• induction of Ifnb1 mRNA by R848, PGN, or Sendai virus is normal in BMDMs and BMDCs

decreased interleukin-6 secretion ( J:251270 )

• TNF- and IL-1beta-triggered transcription of Il6 is significantly impaired in MEFs and BMDCs

• reconstitution of TRIM8 markedly potentiates TNF- and IL-1beta-triggered transcription of Il6 in MEFs

increased interleukin-6 secretion ( J:251270 )

• poly(I:C)- and LPS-induced transcription of Il6 is significantly increased in BMDMs and BMDCs

• poly(I:C)- and LPS- but not Sendai virus (SeV)-, PGN- or R848-induced secretion of IL6 is significantly increased in BMDMs

• poly(I:C)- and LPS- but not SeV-induced transcription of Il6 is markedly increased in MEFs

• reconstitution of TRIM8 markedly inhibits poly(I:C)- and LPS-triggered transcription of Il6 in MEFs

• induction of Il6 mRNA by R848, PGN, or Sendai virus is normal in BMDMs and BMDCs

decreased tumor necrosis factor secretion ( J:251270 )

• TNF- and IL-1beta-triggered transcription of Tnf is significantly impaired in MEFs and BMDCs

• reconstitution of TRIM8 markedly potentiates TNF- and IL-1beta-triggered transcription of Tnf in MEFs

increased tumor necrosis factor secretion ( J:251270 )

• poly(I:C)- and LPS-induced transcription of Tnf is significantly increased in BMDMs and BMDCs

• poly(I:C)- and LPS- but not Sendai virus (SeV)-, PGN- or R848-induced secretion of TNF is significantly increased in BMDMs

• poly(I:C)- and LPS- but not SeV-induced transcription of Tnf is markedly increased in MEFs

• reconstitution of TRIM8 markedly inhibits poly(I:C)- and LPS-triggered transcription of Tnf in MEFs

• induction of Tnf mRNA by R848, PGN, or Sendai virus is normal in BMDMs and BMDCs

increased susceptibility to endotoxin shock ( J:251270 )

• after i.p. injection of LPS, mice exhibit significantly higher serum TNF, IL6 and CCL5 levels at 2 h as well as earlier death onset and a higher percentage of lethality within 42 h post-injection relative to wild-type controls

increased susceptibility to bacterial infection ( J:251270 )

• after oral administration of Salmonella typhimurium, mice exhibit a more severe loss of body weight and septic shock within 6 days post-infection than wild-type controls