Phenotypes associated with this allele

• Allele Symbol: Dnmt3a^tm1Trow
• Allele Name: targeted mutation 1, Jennifer Trowbridge
• Allele ID: MGI:6188116
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Dnmt3a^tm1Trow/Dnmt3a^+ Tg(Mx1-cre)1Cgn/0	B6.Cg-Dnmt3a^tm1Trow Tg(Mx1-cre)1Cgn	MGI:6286132
cn2	Dnmt3a^tm1Trow/Dnmt3a^+ Gt(ROSA)26Sor^tm3(CAG-flpo/ERT2)Alj/Gt(ROSA)26Sor^+ Npm1^tm1Trow/Npm1^+ Tg(Mx1-cre)1Cgn/0	B6.Cg-Gt(ROSA)26Sor^tm3(CAG-flpo/ERT2)Alj Npm1^tm1Trow Dnmt3a^tm1Trow Tg(Mx1-cre)1Cgn	MGI:6286136

Genotype
MGI:6286132

cn1

• Allelic Composition: Dnmt3a^tm1Trow/Dnmt3a⁺; Tg(Mx1-cre)1Cgn/0
• Genetic Background: B6.Cg-Dnmt3a^tm1Trow Tg(Mx1-cre)1Cgn

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

embryo

abnormal multipotent stem cell morphology ( J:272803 )

♀ • 6 months after induction with poly(I:C) (pIpC), multipotent progenitor cell populations increase in percentage and total number in bone marrow

hematopoietic system

increased common myeloid progenitor cell number ( J:272803 )

♀ • 6 months after induction with poly(I:C) (pIpC), multi-lineage, mixed granulocyte/erythroid/macrophage/megakaryocyte (GEMM) colonies increase in percentage and total number in bone marrow

abnormal bone marrow hematopoietic cell morphology ( J:272803 )

♀

increased hematopoietic stem cell number ( J:272803 )

♀ • 6 months after induction with poly(I:C) (pIpC), viable long term hematopoietic stem cells and short term hematopoietic stem cells increase in percentage and total number in bone marrow

Genotype
MGI:6286136

cn2

• Allelic Composition: Dnmt3a^tm1Trow/Dnmt3a⁺; Gt(ROSA)26Sor^{tm3(CAG-flpo/ERT2)Alj}/Gt(ROSA)26Sor⁺; Npm1^tm1Trow/Npm1⁺; Tg(Mx1-cre)1Cgn/0
• Genetic Background: B6.Cg-Gt(ROSA)26Sor^{tm3(CAG-flpo/ERT2)Alj} Npm1^tm1Trow Dnmt3a^tm1Trow Tg(Mx1-cre)1Cgn

hematopoietic system

abnormal bone marrow cell physiology ( J:272803 )

♀ • bone marrow cells transplanted into lethally irradiated recipients and treated with pIpC and tamoxifen results in death in a 100% of animals in a 440 day period

♀ • in 33% of mice death is the result of myeloproliferative disorder (MPD)

♀ • in 67% of mice death is the result of mixed myelodysplastic syndrome and myeloproliferative disorder (MDS/MPD)

♀ • bone marrow cells from MDS/MPD or MPD primary transplants transplanted into sublethally irradiated secondary recipients results in 100% lethality due to acute myeloid leukemia (AML)