Analysis Tools
nervous system
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• spiny projection neurons exhibit dendritic spine-head widths that are larger than in wild-type
• however, mice show normal number of excitatory synapses and dendritic spine number
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• dorsal striatal spiny projection neurons exhibit a 4-fold increase in spontaneous excitatory postsynaptic current (sEPSC) frequency, shorter interevent interval between sEPSCs, and a wider range of sEPSC frequencies at P21, but not at P14, indicating overly active glutamatergic afferents
• however, by P60, sEPSC frequencies are similar to wild-type
• isolating dorsal striatum from cortex normalizes excessive frequency of sEPSCs, indicating that corticostriatal afferents underlie the elevated frequency of sEPSCs
• acute treatment of striatal slices with the LRRK2 kinase inhibitor GNE-7915 reduced the sEPSC frequency to wild-type levels
• however, spiny projection neurons show no differences in membrane capacitance, resting membrane potential, or action potential threshold and mEPSCs are not significantly altered
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• spiny projection neurons exhibit altered distribution of postsynaptic response amplitudes
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• in dorsal striatal spiny projection neurons
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