Analysis Tools
immune system
| N |
• mice show normal formation of high endothelial venules (HEVs), B-cell follicles, and T-cell areas in the lymph nodes (LNs), with no abnormalities in CD4, CD8 or B220 expression in LN cells
• 8-week-old males show no significant differences in the total cell number in spleen, mesenteric LNs (MLNs), inguinal LNs (ILNs) and popliteal LNs (PLNs) relative to wild-type controls
• overall tissue architecture and blood vessel distribution pattern is normal in peripheral LNs
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• immunohistological analysis shows that lymphocyte accumulation within the HEV EC layer of the ILN is only slightly increased (statistically non-significant)
• TEM analysis of HEVs in the ILNs shows more lymphocytes in the HEV EC layer including perivenular channels, occasionally causing narrowing of the luminal surface of HEV ECs
• most accumulated lymphocytes are nested between the ECs and the underlying basal lamina
• most pockets in the HEV EC layer (areas where lymphocytes reside for several minutes before they enter the LN parenchyma) house more lymphocytes (occasionally >10 in a single pocket), whereas wild-type pockets typically house up to 4-5 lymphocytes
• however, after adoptive transfer, both migration of wild-type GFP+ donor cells as well as migration of CMTMR-labeled donor Lpar6-deficient lymphocytes into the ILN and spleen of Lpar6-deficient mice is normal, indicating normal in vivo lymphocyte migration
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hematopoietic system
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• immunohistological analysis shows that lymphocyte accumulation within the HEV EC layer of the ILN is only slightly increased (statistically non-significant)
• TEM analysis of HEVs in the ILNs shows more lymphocytes in the HEV EC layer including perivenular channels, occasionally causing narrowing of the luminal surface of HEV ECs
• most accumulated lymphocytes are nested between the ECs and the underlying basal lamina
• most pockets in the HEV EC layer (areas where lymphocytes reside for several minutes before they enter the LN parenchyma) house more lymphocytes (occasionally >10 in a single pocket), whereas wild-type pockets typically house up to 4-5 lymphocytes
• however, after adoptive transfer, both migration of wild-type GFP+ donor cells as well as migration of CMTMR-labeled donor Lpar6-deficient lymphocytes into the ILN and spleen of Lpar6-deficient mice is normal, indicating normal in vivo lymphocyte migration
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cellular
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• immunohistological analysis shows that lymphocyte accumulation within the HEV EC layer of the ILN is only slightly increased (statistically non-significant)
• TEM analysis of HEVs in the ILNs shows more lymphocytes in the HEV EC layer including perivenular channels, occasionally causing narrowing of the luminal surface of HEV ECs
• most accumulated lymphocytes are nested between the ECs and the underlying basal lamina
• most pockets in the HEV EC layer (areas where lymphocytes reside for several minutes before they enter the LN parenchyma) house more lymphocytes (occasionally >10 in a single pocket), whereas wild-type pockets typically house up to 4-5 lymphocytes
• however, after adoptive transfer, both migration of wild-type GFP+ donor cells as well as migration of CMTMR-labeled donor Lpar6-deficient lymphocytes into the ILN and spleen of Lpar6-deficient mice is normal, indicating normal in vivo lymphocyte migration
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growth/size/body
 N |
• 8-week-old males exhibit normal total body weight relative to wild-type controls
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cardiovascular system
| N |
• i.v. injected FITC-dextran is mostly retained within the vasculature at levels similar to those in wild-type controls
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homeostasis/metabolism
| N |
• high endothelial venules (HEVs) and fibroblastic reticular cells show normal expression of ENPP2/ATX (ectonucleotide pyrophosphatase/phosphodiesterase 2, also known as autotaxin), indicating normal local production of lysophosphatidic acid (LPA)
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