Analysis Tools
reproductive system
| N |
• mutant females show normal ovarian histology at 3 and 18 weeks of age
• average number of ovulated metaphase II (MII) oocytes by 3-week-old mutant females is normal, as determined by a superovulation assay
• in vitro, average germinal vesicle breakdown (GVBD) and first polar body (PB1) emission rates of GV oocytes are similar to those of wild-type females
• mutant males exhibit normal fertility
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• mutant females are sterile
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cellular
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• some normally fertilized zygotes derived from mutant females develop to the 2-cell stage
• none of the embryos derived from mutant females develop to term in wild-type foster mothers
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homeostasis/metabolism
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• decay of maternal mRNA is severely impaired in mutant oocytes; only 14% of mRNAs are degraded at the MII stage while only 64% and 67% are degraded at the 1- and 2-cell stages, respectively
• 63.4% of transcripts that are normally degraded during meiotic maturation and after fertilization fail to be degraded in mutant MII oocytes and zygotes; more maternal transcripts are upregulated than downregulated even at the 2-cell stage, indicating impaired clearance of maternal mRNA
• zygotic genome activation (ZGA) is also impaired: more zygotic transcripts are downregulated than upregulated in mutant 2-cell embryos
• 3-poly(A)-tail shortening of maternal transcripts is blocked or delayed in oocytes and zygotes collected from mutant females
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growth/size/body
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N |
• mutant females show a normal average body weight at 3 weeks of age
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neoplasm
| N |
• mutant mice are viable, do not develop spontaneous tumors, and remain healthy up to 1 yr of age
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