Phenotypes associated with this allele

• Allele Symbol: Trim59^tm1Ryag
• Allele Name: targeted mutation 1, Rongcun Yang
• Allele ID: MGI:6287110
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Trim59^tm1Ryag/Trim59^tm1Ryag	involves: C57BL/6J * C57BL/6NTac	MGI:6287119

Genotype
MGI:6287119

hm1

• Allelic Composition: Trim59^tm1Ryag/Trim59^tm1Ryag
• Genetic Background: involves: C57BL/6J * C57BL/6NTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality, complete penetrance ( J:272362 )

• empty yolk sacs are observed at E8.5 and no homozygous mutant embryos are detected at E9.5

embryo

abnormal embryo development ( J:272362 )

• early embryo developmental defects from the blastocyst to the gastrula stage

abnormal germ layer development ( J:272362 )

• no normal three germ layers are observed at E7.5

abnormal endoderm development ( J:272362 )

• Cer1 expression in the definitive endoderm is markedly decreased at E7.5

failure to gastrulate ( J:272362 )

• expression of primary germ layer formation-associated genes including Brachyury, Lefty2, Cer1, Otx2, Wnt3, and Bmp4 is reduced at E6.5 and E7.5

embryonic growth retardation ( J:272362 )

• embryos appear developmentally delayed at E6.5 and E7.5

small embryonic epiblast ( J:272362 )

• a contracted embryonic epiblast is observed at E6.5

absent mesoderm ( J:272362 )

• no mesoderm is observed at E6.5; expression of Lefty2 (a nascent mesoderm marker) is absent

abnormal embryonic neuroepithelium morphology ( J:272362 )

• Otx2 expression in the anterior neuroectoderm (ANE) is markedly decreased at E7.5

failure of primitive streak formation ( J:272362 )

• no Brachyury expression is detected at E6.5 and E7.5, indicating failure of primitive streak formation

abnormal inner cell mass morphology ( J:272362 )

• at E6.5 and E7.5, the expression region of Oct4 is significantly decreased, indicating abnormal ICM differentiation

• F-actin assembly is perturbed during inner cell mass differentiation at E3.5 and E4.5

absent amnion ( J:272362 )

• no amnion is observed at E7.5

absent amniotic cavity ( J:272362 )

• no amniotic cavity is observed at E7.5

absent extraembryonic coelom ( J:272362 )

• no extraembryonic coelom is observed at E7.5

absent extraembryonic ectoderm ( J:272362 )

• no extraembryonic ectoderm is observed at E7.5

nervous system

abnormal embryonic neuroepithelium morphology ( J:272362 )

• Otx2 expression in the anterior neuroectoderm (ANE) is markedly decreased at E7.5

growth/size/body

embryonic growth retardation ( J:272362 )

• embryos appear developmentally delayed at E6.5 and E7.5

reproductive system

empty decidua capsularis ( J:272362 )

• some empty swollen decidua are detected at E8.5