All Phenotypes Agbl5<tm1.1Ics> MGI Mouse

reproductive system phenotype ( J:271687 )

N	• male mice are fertile, unlike male Agbl5^tm1.2Ics homozygotes

abnormal sperm axoneme morphology ( J:271687 )

• splayed axonemes are observed

hairpin sperm flagellum ( J:271687 )

• epididymal sperm show malformed sperm tails with hairpin bending (back-folded initial segments)

oligozoospermia ( J:271687 )

• caudal epididymal sperm count is markedly reduced

detached acrosome ( J:271687 )

abnormal sperm nucleus morphology ( J:271687 )

• abnormally-shaped sperm nuclei and nuclear inclusions are observed

abnormal spermatid morphology ( J:271687 )

• round and early elongating spermatids exhibit highly glutamylated microtubules

• elongating spermatids have curly flagella and misshapen nuclei

• number of elongating spermatids with supernumerary (>2) basal bodies is increased

abnormal manchette perinuclear ring morphology ( J:271687 )

• absence of the perinuclear ring

abnormal seminiferous tubule morphology ( J:271687 )

• spermatids are mislocalized at different stages throughout the different regions of the seminiferous tubules

• a reduced number of mature spermatozoa are found in the lumen of the tubules

• however, seminiferous tubule size is normal

abnormal spermiogenesis ( J:271687 )

• testis tissue is disordered with spermatids of different developmental stages found mislocalized

• various defects in spermiogenesis are observed at all stages from the early elongating spermatid stage to the condensed spermatid stage, similar to the ultrastructural phenotypes seen in male Agbl5^tm1.2Ics homozygotes

• vacuolization of the cytoplasm and abnormal accumulation of cytoplasm in sperm cells with highly condensed nucleus is observed

• however, assembly of sperm flagella is observed indicating partial maturation of sperm cells, unlike in male Agbl5^tm1.2Ics homozygotes

abnormal manchette assembly ( J:271687 )

• disordered manchette microtubules and absence of the perinuclear ring and circumferential groove are observed

abnormal cauda epididymis morphology ( J:271687 )

• a decreased number of mature spermatozoa and an increased number of precursor cells are found in the cauda epididymis