Phenotypes associated with this allele

• Allele Symbol: Bcl6^tm1Gdba
• Allele Name: targeted mutation 1, Grant D Barish
• Allele ID: MGI:6293908
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Bcl6^tm1Gdba/Bcl6^tm1Gdba Tg(ACTA1-rtTA,tetO-cre)102Monk/0	involves: C3H * C57BL/6	MGI:6294154
cn2	Bcl6^tm1Gdba/Bcl6^tm1Gdba Tg(Adipoq-cre)1Evdr/0	involves: C57BL/6 * FVB/NJ	MGI:6294151

Genotype
MGI:6294154

cn1

• Allelic Composition: Bcl6^tm1Gdba/Bcl6^tm1Gdba; Tg(ACTA1-rtTA,tetO-cre)102Monk/0
• Genetic Background: involves: C3H * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

adipose tissue

increased white adipose tissue amount ( J:271078 )

• dox-treated mice develop increased white adipose tissue in all depots, including the inguinal, interscapular, perigonadal, perinephric, and interscarpular adipose tissues but not brown adipose tissue

increased body fat mass ( J:271078 )

• mice treated with doxycycline (dox) for 7 days at 8 weeks of age exhibit a higher fat mass within 4 weeks after dox treatment

increased white fat cell size ( J:271078 )

• adipocyte size is increased in all white adipose tissue depots of dox-treated mice

growth/size/body

increased body fat mass ( J:271078 )

• mice treated with doxycycline (dox) for 7 days at 8 weeks of age exhibit a higher fat mass within 4 weeks after dox treatment

Genotype
MGI:6294151

cn2

• Allelic Composition: Bcl6^tm1Gdba/Bcl6^tm1Gdba; Tg(Adipoq-cre)1Evdr/0
• Genetic Background: involves: C57BL/6 * FVB/NJ

growth/size/body

increased body fat mass ( J:271078 )

• mice fed a regular chow exhibit increased mass and percentage of fat

• however, body weight of regular chow fed mice is normal

increased susceptibility to diet-induced obesity ( J:271078 )

• mice fed a high-fat diet exhibit a greater increase in total fat mass than controls fed the same diet

• adiposity in high-fat diet fed mice is due to increased subcutaneous adipose tissue

adipose tissue

increased subcutaneous adipose tissue amount ( J:271078 )

• expanded fat mass in regular chow-fed mice is due to a 2-fold increase in subcutaneous, but not perigonadal or brown, adipose tissue

• adiposity in high-fat diet fed mice is due to increased subcutaneous adipose tissue

increased body fat mass ( J:271078 )

• mice fed a regular chow exhibit increased mass and percentage of fat

• however, body weight of regular chow fed mice is normal

increased white fat cell size ( J:271078 )

• subcutaneous adipocyte diameters are larger, with this size difference limited to subcutaneous and not perigonadal adipose tissue

• subcutaneous adipocytes of high-fat fed mice exhibit larger size, with nearly 50% of cells having greater than 60 micron diameters compared to 25% of adipocytes in controls

• however, there is no evidence of hypercellularity in adipose tissues

abnormal gonadal fat pad morphology ( J:271078 )

• perigonadal adipose tissue is reduced by 30% compared to controls in high-fat diet fed mice

• perigonadal adipose tissue of high-fat diet fed mice has increased interstitial cell infiltration and crown-like structures

decreased adipocyte glucose uptake ( J:271078 )

• mice exhibit decreased insulin-stimulated glucose uptake into brown adipose tissue

increased adipocyte glucose uptake ( J:271078 )

• mice exhibit increased insulin-stimulated glucose uptake into perigonadal adipose tissue

white adipose tissue inflammation ( J:271078 )

• perigonadal adipose tissue of high-fat diet fed mice has increased interstitial cell infiltration and crown-like structures

cellular

decreased adipocyte glucose uptake ( J:271078 )

• mice exhibit decreased insulin-stimulated glucose uptake into brown adipose tissue

increased adipocyte glucose uptake ( J:271078 )

• mice exhibit increased insulin-stimulated glucose uptake into perigonadal adipose tissue

increased skeletal muscle cell glucose uptake ( J:271078 )

• mice exhibit increased insulin-stimulated glucose uptake in gastrocnemius and vastus lateralis muscles

homeostasis/metabolism

increased susceptibility to diet-induced obesity ( J:271078 )

• mice fed a high-fat diet exhibit a greater increase in total fat mass than controls fed the same diet

• adiposity in high-fat diet fed mice is due to increased subcutaneous adipose tissue

decreased fasting circulating glucose level ( J:271078 )

• both regular chow and high-fat diet fed mice exhibit reduced fasting glucose levels

decreased circulating insulin level ( J:271078 )

• both regular chow and high-fat diet fed mice exhibit reduced fasting insulin levels

• both pre-clamp and clamped plasma insulin levels are lower

increased circulating leptin level ( J:271078 )

• levels of leptin are higher in chow-fed, but not high-fat diet fed, mice

decreased respiratory quotient ( J:271078 )

• mice exhibit no differences in energy expenditure although respiratory exchange ratio is lower at some time points

impaired gluconeogenesis ( J:271078 )

• rate of endogenous glucose production is reduced during hyperinsulinemic-euglycemic clamp, indicating that gluconeogenesis is more sensitive to insulin-mediated suppression

increased insulin sensitivity ( J:271078 )

• insulin resistance is reduced in both regular diet and high-fat diet fed mice, indicating improved glucose metabolism

• high-fat diet fed mice require glucose infusion rates nearly double those of controls to maintain steady-state glucose levels, indicating enhanced insulin sensitivity

increased circulating adiponectin level ( J:271078 )

• about 30% increase in serum levels of adiponectin in regular chow-fed or high-fat diet fed mice

abnormal lipid level ( J:271078 )

• 2.5-fold elevation of fatty acid esters of hydroxy fatty acid (FAHFAs) levels in serum, including the 16:0H18:0 form (PAHSA)

• levels of many other lipids, including triglyceride-associated fatty acyl chains, total triglycerides, ceramides, and several glycerophospholipids are reduced in serum

• subcutaneous adipose tissue shows twice as high levels of insulin-sensitizing lipokines known as FAHFs and C16:1n7-palmitoleate and increased levels of other triglyceride-associated fatty acyl chains and total triglycerides, various membrane-associated and signaling glycerophospholipids, and sphingomyelin

decreased circulating free fatty acids level ( J:271078 )

• fasting plasma free fatty acid levels are normal, but clamped levels are lower, indicating enhanced insulin-mediated suppression of lipolysis

decreased circulating triglyceride level ( J:271078 )

• total triglyceride levels are reduced in serum

decreased ceramide level ( J:271078 )

• serum ceramide levels are reduced

decreased liver triglyceride level ( J:271078 )

• liver triglyceride content is reduced by 48% in chow-fed and 44% in high-fat fed mice

increased lipogenesis ( J:271078 )

• mice exhibit a 2-fold increase in rates of de novo synthesis of triglyceride-bound palmitate and triglyceride-bound glycerol in the subcutaneous adipose tissue indicating increase in de novo lipogenesis

• however, mice exhibit normal fatty acid oxidation and acute lipolytic responses to beta adrenergic agonists

immune system

white adipose tissue inflammation ( J:271078 )

• perigonadal adipose tissue of high-fat diet fed mice has increased interstitial cell infiltration and crown-like structures

integument

increased subcutaneous adipose tissue amount ( J:271078 )

• expanded fat mass in regular chow-fed mice is due to a 2-fold increase in subcutaneous, but not perigonadal or brown, adipose tissue

• adiposity in high-fat diet fed mice is due to increased subcutaneous adipose tissue

liver/biliary system

decreased liver triglyceride level ( J:271078 )

• liver triglyceride content is reduced by 48% in chow-fed and 44% in high-fat fed mice

decreased liver weight ( J:271078 )

• liver weight is reduced by 13% in chow-fed and 20% in high-fat fed mice

decreased susceptibility to diet-induced hepatic steatosis ( J:271078 )

• mice fed a high-fat diet show a reduction in steatosis compared to wild-type mice fed the same diet

muscle

increased skeletal muscle cell glucose uptake ( J:271078 )

• mice exhibit increased insulin-stimulated glucose uptake in gastrocnemius and vastus lateralis muscles