All Phenotypes Tg(Adipoq-rtTA)2Zvw MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Xbp1^tm2Glm/Xbp1^tm2Glm Tg(Adipoq-rtTA)2Zvw/0 Tg(tetO-cre)1Jaw/0	involves: 129 * 129S6/SvEvTac * C57BL/6 * C57BL/6N	MGI:6376195
cn2	Cad^{tm1c(KOMP)Wtsi}/Cad^{tm1c(KOMP)Wtsi} Tg(Adipoq-rtTA)2Zvw/0 Tg(tetO-cre)1Jaw/0	involves: 129 * C57BL/6 * C57BL/6N	MGI:6376235
cn3	Cad^{tm1c(KOMP)Wtsi}/Cad^{tm1c(KOMP)Wtsi} Tg(Adipoq-rtTA)2Zvw/0 Tg(tetO-cre)1Jaw/0 Tg(tetO-Xbp1_is)#Pesch/0	involves: 129 * C57BL/6 * C57BL/6N * FVB/N	MGI:6376231
cx4	Tg(tetO-Xbp1_is)#Pesch/0 Tg(Adipoq-rtTA)2Zvw/0	involves: C57BL/6 * C57BL/6N * FVB/N	MGI:6376191
cx5	Lep^ob/Lep^ob Tg(tetO-Xbp1_is)#Pesch/0 Tg(Adipoq-rtTA)2Zvw/0	involves: C57BL/6 * C57BL/6N * FVB/N	MGI:6376200

Allelic Composition	Xbp1^tm2Glm/Xbp1^tm2Glm Tg(Adipoq-rtTA)2Zvw/0 Tg(tetO-cre)1Jaw/0
Genetic Background	involves: 129 * 129S6/SvEvTac * C57BL/6 * C57BL/6N

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Adipoq-rtTA)2Zvw mutation (1 available)
Tg(tetO-cre)1Jaw mutation (6 available)
Xbp1^tm2Glm mutation (0 available); any Xbp1 mutation (29 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

homeostasis/metabolism

abnormal nucleotide metabolism ( J:260830 )

• the 24 hour fasting-induced increase in plasma uridine that is seen in wild-type mice is reduced in doxycycline (Dox)-treated mutant mice

• however, mice do not show a reduced lipolysis response to the Beta3 adrenergic receptor agonist CL316,243

adipose tissue

increased body fat mass ( J:260830 )

• mice that are switched to a Dox high-fat diet at 69 weeks of age show a higher fat mass than controls 4 weeks after the diet switch

• however, Dox-treated mice fed a high-fat diet do not show a difference in body weight gain from wild-type mice fed the same diet

growth/size/body

increased body fat mass ( J:260830 )

• mice that are switched to a Dox high-fat diet at 69 weeks of age show a higher fat mass than controls 4 weeks after the diet switch

• however, Dox-treated mice fed a high-fat diet do not show a difference in body weight gain from wild-type mice fed the same diet

Allelic Composition	Cad^{tm1c(KOMP)Wtsi}/Cad^{tm1c(KOMP)Wtsi} Tg(Adipoq-rtTA)2Zvw/0 Tg(tetO-cre)1Jaw/0
Genetic Background	involves: 129 * C57BL/6 * C57BL/6N
Cell Lines	EPD0282_2_F10

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cad^{tm1c(KOMP)Wtsi} mutation (0 available); any Cad mutation (75 available)
Tg(Adipoq-rtTA)2Zvw mutation (1 available)
Tg(tetO-cre)1Jaw mutation (6 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

adipose tissue

decreased white fat cell size ( J:260830 )

• mice show a moderate size reduction of adipocytes in subcutaneous white adipose tissue (sWAT) when fed a Dox-containing diet

• however, no differences in epididymal white adipose tissue (eWAT) or interscapular brown adipose tissue (BAT) adipocyte size is seen

growth/size/body

growth/size/body region phenotype ( J:260830 )

N	• mice do not show differences in bodyweight gain from wild-type mice when treated with doxycycline (Dox)

Allelic Composition	Cad^{tm1c(KOMP)Wtsi}/Cad^{tm1c(KOMP)Wtsi} Tg(Adipoq-rtTA)2Zvw/0 Tg(tetO-cre)1Jaw/0 Tg(tetO-Xbp1_is)#Pesch/0
Genetic Background	involves: 129 * C57BL/6 * C57BL/6N * FVB/N
Cell Lines	EPD0282_2_F10

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

growth/size/body

weight loss ( J:260830 )

• mice continue to increase their body weight when switched to a doxycycline (Dox)-containing diet, although not at the same extent as wild-type mice thus showing a partial reversal of weight loss

adipose tissue

adipose tissue phenotype ( J:260830 )

• size of adipocytes is epididymal white adipose tissue (eWAT), subcutaneous white adipose tissue (sWAT), and in interscapular brown adipose tissue (BAT)is normal in Dox-treated mice

• adipose tissue uridine content is not increased and thus rescued in Dox-treated mice compared to Tg(tetO-Xbp1_is)#Pesch/0 Tg(Adipoq-rtTA)2Zvw/0 mice

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:260830 )

N	• adipose tissue uridine content is not increased and thus rescued in Dox-treated mice compared to Tg(tetO-Xbp1_is)#Pesch/0 Tg(Adipoq-rtTA)2Zvw/0 mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

growth/size/body

decreased body fat mass ( J:260830 )

• weight loss in doxycycline (Dox)-treated mice is mainly due to a reduction of fat mass

weight loss ( J:260830 )

• mice start to lose body weight immediately upon switching to Dox-containing diet

decreased susceptibility to diet-induced obesity ( J:260830 )

• Dox-treated mice fed a high-fat diet show a decrease in body weight and lower overall adiposity compared to wild-type mice

homeostasis/metabolism

increased fatty acid oxidation ( J:260830 )

• 6 days after the switch to Dox chow, fatty acid oxidation rates are elevated in sWAT

• 90 days after the switch to Dox chow, fatty acid oxidation rates are elevated in both sWAT and BAT

• however, lipid uptake rates are normal

• however, fatty acid or ketone bodies in circulation upon fasting are not affected in Dox-treated mice

decreased susceptibility to diet-induced obesity ( J:260830 )

• Dox-treated mice fed a high-fat diet show a decrease in body weight and lower overall adiposity compared to wild-type mice

decreased body temperature ( J:260830 )

• mice maintain their body temperature slightly but significantly lower than wild-type mice by day 10 of Dox exposure

• however, Dox-treated mice show no difference in food intake

increased carbon dioxide production ( J:260830 )

• mice exhibit higher rates of carbon dioxide release under fed, fasted, and refed conditions within 10 days after the switch to Dox chow

increased oxygen consumption ( J:260830 )

• mice exhibit higher rates of oxygen consumption under fed, fasted, and refed conditions within 10 days after the switch to Dox chow

increased basal metabolism ( J:260830 )

• mice exhibit higher rates of oxygen consumption and carbon dioxide release under fed, fasted, and refed conditions within 10 days after the switch to Dox chow, indicating increased metabolic rate

• heat production is higher under all conditions tested in Dox-treated mice

abnormal nucleotide metabolism ( J:260830 )

• upon induction with doxycycline (Dox), mice increase plasma uridine levels 2-fold and maintain elevated levels as long as they are kept on Dox

• Dox-treated mice exhibit higher uridine concentrations in epididymal white adipose tissue (eWAT), subcutaneous white adipose tissue (sWAT), and in interscapular brown adipose tissue (BAT)

• adipocytes grown in culture in the presence of Dox show an initial consumption of uridine by the cells similarly to wild-type cells but subsequently show a greater net release of uridine into the culture medium than wild-type cells

• suppression of uridine release by PALA, an inhibitor of de novo pyrimidine synthesis, is abolished in mature adipocytes

adipose tissue

decreased body fat mass ( J:260830 )

• weight loss in doxycycline (Dox)-treated mice is mainly due to a reduction of fat mass

decreased brown adipose tissue mass ( J:260830 )

• smaller BAT weight in Dox-treated mice

decreased fat cell size ( J:260830 )

• adipocyte size is reduced in Dox-treated mice

abnormal white adipose tissue morphology ( J:260830 )

• Dox-treated mice exhibit less fibrosis and inflammation in eWAT at 40 weeks of age

decreased white adipose tissue mass ( J:260830 )

• smaller WAT weight in Dox-treated mice

cellular

increased fatty acid oxidation ( J:260830 )

• 6 days after the switch to Dox chow, fatty acid oxidation rates are elevated in sWAT

• 90 days after the switch to Dox chow, fatty acid oxidation rates are elevated in both sWAT and BAT

• however, lipid uptake rates are normal

• however, fatty acid or ketone bodies in circulation upon fasting are not affected in Dox-treated mice

Allelic Composition	Lep^ob/Lep^ob Tg(tetO-Xbp1_is)#Pesch/0 Tg(Adipoq-rtTA)2Zvw/0
Genetic Background	involves: C57BL/6 * C57BL/6N * FVB/N

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lep^ob mutation (5 available); any Lep mutation (21 available)
Tg(Adipoq-rtTA)2Zvw mutation (1 available)
Tg(tetO-Xbp1_is)#Pesch mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

growth/size/body

decreased susceptibility to diet-induced obesity ( J:260830 )

• doxycycline (Dox)-treated mice fed a high-fat diet show a slower weight gain compared to single Lep^ob homozygous mice but do not show an actual net weight loss

• Dox-treated mice fed a high-fat diet show a reduction in fat mass gain compared to Lep^ob homozygous mice

homeostasis/metabolism

decreased susceptibility to diet-induced obesity ( J:260830 )

• doxycycline (Dox)-treated mice fed a high-fat diet show a slower weight gain compared to single Lep^ob homozygous mice but do not show an actual net weight loss

• Dox-treated mice fed a high-fat diet show a reduction in fat mass gain compared to Lep^ob homozygous mice

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