Phenotypes associated with this allele

• Allele Symbol: Nsl1^em1(IMPC)J
• Allele Name: endonuclease-mediated mutation 1, Jackson
• Allele ID: MGI:6317375
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Nsl1^em1(IMPC)J/Nsl1^em1(IMPC)J	C57BL/6NJ-Nsl1^em1(IMPC)J/Mmjax	MGI:6480533
ht2	Nsl1^em1(IMPC)J/Nsl1^+	C57BL/6NJ-Nsl1^em1(IMPC)J/Mmjax	MGI:6492990

Genotype
MGI:6480533

hm1

• Allelic Composition: Nsl1^em1(IMPC)J/Nsl1^em1(IMPC)J
• Genetic Background: C57BL/6NJ-Nsl1^em1(IMPC)J/Mmjax

Data Sources

IMPC Data for Nsl1

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Nsl1^em1(IMPC)J/Nsl1^em1(IMPC)J mice exhibit embryonic lethality, with embryos recovered at E3.5 as morulae but not at E7.5. Mutants fail to hatch from the zona pellucida and are dead after 72hr in vitro, never forming blastocysts.

embryo

failure of blastocyst formation ( J:279207 )

• in vitro, embryos fail to form blastocysts and are dead after 3 days in culture

failure of blastocyst to hatch from the zona pellucida ( J:279207 )

• in vitro, embryos fail to hatch from the zona pellucida after 3 days in culture

mortality/aging

embryonic lethality prior to organogenesis ( J:211773 )

IMPC - JAX

preweaning lethality, complete penetrance ( J:211773 )

IMPC - JAX

embryonic lethality prior to organogenesis, complete penetrance ( J:279207 )

• morula-like embryos are present in approximate Mendelian ratios at E3.5 (34% versus expected 25%, n=29); no homozygous embryos are recovered at E7.5

• however, excess resorption sites suggest that embryos elicit a decidual response in vivo

Genotype
MGI:6492990

ht2

• Allelic Composition: Nsl1^em1(IMPC)J/Nsl1⁺
• Genetic Background: C57BL/6NJ-Nsl1^em1(IMPC)J/Mmjax

Data Sources

IMPC Data for Nsl1

behavior/neurological

increased grip strength ( J:211773 )

♀

IMPC - JAX

hematopoietic system

decreased leukocyte cell number ( J:211773 )

IMPC - JAX

immune system

decreased leukocyte cell number ( J:211773 )

IMPC - JAX