All Phenotypes H3f4<em1Osb> MGI Mouse

reproductive system phenotype ( J:258191 )

N	• females are fertile

abnormal spermatogonia morphology ( J:258191 )

• numbers of c-Kit-positive differentiating spermatogonia are significantly decreased

• however, numbers of promyelocytic leukemia zinc finger (PLZF)-positive undifferentiated spermatogonia are normal

decreased male germ cell number ( J:258191 )

• numbers of TRA98-positive germ cells start to decline from P10; this defect becomes more severe at P12

• by P14, germ cells are eventually lost by apoptosis, as shown by TUNEL assays

small testis ( J:258191 )

• testes are strikingly smaller

abnormal spermatogenesis ( J:258191 )

• the first wave of spermatogenesis is already impaired at 4 weeks of age

azoospermia ( J:258191 )

• azoospermia stemming from spermatogenic failure

abnormal spermatid morphology ( J:258191 )

• elongating and acrosome-positive spermatids are absent

abnormal spermatocyte morphology ( J:258191 )

• numbers of gamma-H2A.X histone variant-positive spermatocytes are significantly decreased

abnormal male meiosis ( J:258191 )

• most seminiferous tubules lack synaptonemal complex protein 3 (SCP3)-positive cells suggesting a defect before meiosis

• male germ cells are unable to enter meiosis

• caudae epididymides are transparent and lack spermatozoa

male infertility ( J:258191 )

• males are sterile