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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Plekhf1tm1.1Caox
targeted mutation 1.1, Xuetao Cao
MGI:6343232
Summary 1 genotype
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Plekhf1tm1.1Caox/Plekhf1tm1.1Caox
Lyz2tm1(cre)Ifo/Lyz2+
B6.Cg-Plekhf1tm1.1Caox Lyz2tm1(cre)Ifo MGI:6360685


Genotype
MGI:6360685
cn1
Allelic
Composition
Plekhf1tm1.1Caox/Plekhf1tm1.1Caox
Lyz2tm1(cre)Ifo/Lyz2+
Genetic
Background
B6.Cg-Plekhf1tm1.1Caox Lyz2tm1(cre)Ifo
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lyz2tm1(cre)Ifo mutation (14 available); any Lyz2 mutation (40 available)
Plekhf1tm1.1Caox mutation (0 available); any Plekhf1 mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• after stimulation with heat-killed and PI (propidium iodide)-labeled E. coli for 1 h, peritoneal macrophages endocytose significantly less E. coli than controls macrophages; endocytosed E. coli do not colocalize with Cav1, unlike in control macrophages
• 1 h after infection macrophages show significantly less endocytosis of viable E. coli or S. aureus than control macrophages, as measured by CFUs
• after incubation with Zymosan or latex beads, mean fluorescence intensity of bone marrow-derived macrophages (BMDMs) is significantly lower than that of control macrophages
• upon LPS stimulation, % of surface TLR4 on BMDMs is significantly higher than that on control macrophages, indicating impaired LPS-activated endocytosis of TLR4

immune system
• after exposure to E. coli, the % of live E. coli counts to initially internalized counts is significantly higher than that in control macrophages, suggesting that bactericidal capacity of macrophages is impaired
• serum IFN-beta levels are significantly reduced at 4 and 8 h after i.p. injection with E. coli
• serum IL-6 levels are significantly reduced at 4 and 8 h after i.p. injection with E. coli
• serum TNFalpha levels are significantly reduced at 4 and 8 h after i.p. injection with E. coli
• upon E. coli or LPS challenge, mice show significantly reduced production of the inflammatory cytokines TNFalpha, IFN-beta, and IL-6 in serum relative to control mice
• upon E. coli or LPS stimulation, macrophages show deceased production of TNFalpha, IFN-beta, and IL-6 relative to control macrophages
• mice are more susceptible to i.p. E. coli infection with significantly higher bacterial loads in spleen and liver, reduced production of TNFalpha, IFN-beta, and IL-6 in serum, and decreased infiltration of inflammatory cells in lung relative to similarly infected control mice
• after i.p. injection with E. coli, all mice die by 3 days after challenge whereas most control mice survive to 5 days post infection

homeostasis/metabolism
• serum IFN-beta levels are significantly reduced at 4 and 8 h after i.p. injection with E. coli
• serum IL-6 levels are significantly reduced at 4 and 8 h after i.p. injection with E. coli
• serum TNFalpha levels are significantly reduced at 4 and 8 h after i.p. injection with E. coli

hematopoietic system
• after exposure to E. coli, the % of live E. coli counts to initially internalized counts is significantly higher than that in control macrophages, suggesting that bactericidal capacity of macrophages is impaired

mortality/aging
• after i.p. injection with E. coli, all mice die by 3 days after challenge whereas most control mice survive to 5 days post infection





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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory