Phenotypes associated with this allele

• Allele Symbol: Lpin3^tm1Reue
• Allele Name: targeted mutation 1, Karen Reue
• Allele ID: MGI:6343243
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Lpin3^tm1Reue/Lpin3^tm1Reue	involves: 129P2/OlaHsd * BALB/cByJ * C57BL/6J	MGI:6381354
hm2	Lpin3^tm1Reue/Lpin3^tm1Reue	involves: 129P2/OlaHsd * C57BL/6J	MGI:6381352
cx3	Lpin1^fld/Lpin1^fld Lpin3^tm1Reue/Lpin3^tm1Reue	involves: 129P2/OlaHsd * BALB/cByJ * C57BL/6J	MGI:6381353

Genotype
MGI:6381354

hm1

• Allelic Composition: Lpin3^tm1Reue/Lpin3^tm1Reue
• Genetic Background: involves: 129P2/OlaHsd * BALB/cByJ * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

decreased body weight ( J:220917 )

• males exhibit lowered body weight while females show a trend toward lower body weight

• however, fat pad size is not altered

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:220917 )

N • mice exhibit normal blood chemistry, showing normal levels of alanine aminotransferase, aspartate aminotransferase, total bilirubin, albumin, gamma-glutamyl transpeptidase, blood urea nitrogen, inorganic phosphorus, creatine kinase, cholesterol, total protein, glucose, and amylase

decreased triglyceride level ( J:220917 )

• mice retain 40-80% of wild-type triacylglycerol levels in adipose tissue indicating a small decrease in levels

abnormal enzyme/coenzyme activity ( J:220917 )

• phosphatidate phosphatase enzyme activity is reduced by 85% in adipose tissue

• phosphatidate phosphatase enzyme activity is also reduced in the spleen, kidney and liver

Genotype
MGI:6381352

hm2

• Allelic Composition: Lpin3^tm1Reue/Lpin3^tm1Reue
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

growth/size/body

decreased body weight ( J:220917 )

• at 14-16 months of age, mice have slightly reduced body weights, however tissue weights are normal

• however, body weight and tissue weight on a chow diet is normal in younger mice, no differences are seen on a high-fat diet, and plasma lipid levels are normal at 14-16 months of age

adipose tissue

abnormal adipose tissue development ( J:220917 )

• a smaller proportion of stromal vascular cells from fat pads accumulate lipid droplets and the droplets are smaller and expression of genes that promote adipogenesis is attenuated in adipocytes, indicating reduced capacity for adipocyte development

abnormal fat cell differentiation ( J:220917 )

• primary preadipocytes exhibit impaired adipocyte differentiation in vitro

cellular

abnormal fat cell differentiation ( J:220917 )

• primary preadipocytes exhibit impaired adipocyte differentiation in vitro

Genotype
MGI:6381353

cx3

• Allelic Composition: Lpin1^fld/Lpin1^fld; Lpin3^tm1Reue/Lpin3^tm1Reue
• Genetic Background: involves: 129P2/OlaHsd * BALB/cByJ * C57BL/6J

growth/size/body

decreased body weight ( J:220917 )

• decrease in body weight due to less adipose tissue, however liver, kidney, heart, and spleen weights are normal

liver/biliary system

decreased liver triglyceride level ( J:220917 )

adipose tissue

abnormal fat pad morphology ( J:220917 )

• fat pad sizes are smaller than in single Lpin1^fld homozygotes

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:220917 )

N • mice exhibit normal blood chemistry, showing normal levels of alanine aminotransferase, aspartate aminotransferase, total bilirubin, albumin, gamma-glutamyl transpeptidase, blood urea nitrogen, inorganic phosphorus, creatine kinase, cholesterol, total protein, glucose, and amylase

decreased triglyceride level ( J:220917 )

• triacylglycerol levels are undetectable within adipose tissue and are lower in spleen, kidney, and liver

decreased liver triglyceride level ( J:220917 )

abnormal enzyme/coenzyme activity ( J:220917 )

• phosphatidate phosphatase enzyme activity is reduced in adipose tissue, spleen kidney, liver, heart, skeletal muscle, brain, and lung