Phenotypes associated with this allele

• Allele Symbol: Col1a1^{tm11(tetO-Nup88)Jvd}
• Allele Name: targeted mutation 11, Jan MA van Deursen
• Allele ID: MGI:6343326
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Col1a1^tm11(tetO-Nup88)Jvd/Col1a1^+	involves: 129S4/SvJae * C57BL/6	MGI:6377632
cx2	Apc^Min/Apc^+ Col1a1^tm11(tetO-Nup88)Jvd/Col1a1^+	involves: 129S4/SvJae * C57BL/6 * C57BL/6J	MGI:6377634

Genotype
MGI:6377632

ht1

• Allelic Composition: Col1a1^{tm11(tetO-Nup88)Jvd}/Col1a1⁺
• Genetic Background: involves: 129S4/SvJae * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

aneuploidy ( J:231166 )

• dox-treated MEFs exhibit higher aneuploidy rates

• splenocytes from 5 month old dox-treated mice show about 4-fold higher rates of aneuploidy than control splenocytes

• lung tissues from 5 month old dox-treated mice are more aneuploid than control lung tissues

abnormal cell cycle checkpoint function ( J:231166 )

• dox-treated MEFs exhibit mitotic checkpoint defects

abnormal mitosis ( J:231166 )

• dox-treated MEFs have higher rates of chromatin bridges and lagging chromosomes, indicating chromosomal segregation defects

• dox-treated MEFs exhibit increased incomplete centrosome separation

• MEFs treated with the PLK inhibitor BI2536 show restoration of normal centrosome separation

abnormal mitotic spindle morphology ( J:231166 )

• dox-treated MEFs exhibit more frequent spindle geometry defects showing mitotic spindle asymmetry

chromosomal instability ( J:231166 )

neoplasm

increased tumor incidence ( J:231166 )

• mice administered doxycycline (dox) beginning at weaning are prone to spontaneous tumors, particularly lung tumors, by 14 months of age, with 56% of mice having at least one neoplastic lesion compared to 21% of controls

increased lung tumor incidence ( J:231166 )

• in dox-treated mice

respiratory system

increased lung tumor incidence ( J:231166 )

• in dox-treated mice

Genotype
MGI:6377634

cx2

• Allelic Composition: Apc^Min/Apc⁺; Col1a1^{tm11(tetO-Nup88)Jvd}/Col1a1⁺
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * C57BL/6J

digestive/alimentary system

increased colon tumor incidence ( J:231166 )

• doxycycline (dox)-treated mice show increased incidence of colon tumors compared to single Apc^Min heterozygotes, but tumor multiplicity or size is unaffected and there is no difference in the multiplicity of small intestinal polyps

• mice in which dox is discontinued 30 days before analysis, show the same incidence of colon tumors as in mice with continuous administration of dox

neoplasm

increased colon tumor incidence ( J:231166 )

• doxycycline (dox)-treated mice show increased incidence of colon tumors compared to single Apc^Min heterozygotes, but tumor multiplicity or size is unaffected and there is no difference in the multiplicity of small intestinal polyps

• mice in which dox is discontinued 30 days before analysis, show the same incidence of colon tumors as in mice with continuous administration of dox