Phenotypes associated with this allele

• Allele Symbol: Apmap^tm1.2Jgbs
• Allele Name: targeted mutation 1.2, Juliane Bogner-Strauss
• Allele ID: MGI:6343493
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Apmap^tm1.2Jgbs/Apmap^tm1.2Jgbs	B6.129P2-Apmap^tm1.2Jgbs	MGI:6368194

Genotype
MGI:6368194

hm1

• Allelic Composition: Apmap^tm1.2Jgbs/Apmap^tm1.2Jgbs
• Genetic Background: B6.129P2-Apmap^tm1.2Jgbs

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

adipose tissue

decreased brown adipose tissue mass ( J:247647 )

♂ • after 22 weeks on a high-fat diet (HFD), wet weight of brown adipose tissue (BAT) is significantly lower than that in wild-type controls

♂ • however, BAT morphology and cold tolerance are normal on a HFD

abnormal white adipose tissue morphology ( J:247647 )

♂ • after 22 weeks on a HFD, mRNA expression of profibrotic collagens (Col1a1, -3a1, -6a1 and -6a2), total tissue collagen content, and Trichrome staining for collagen are significantly decreased in epididymal WAT, indicating reduced fibrotic potential

increased white fat cell number ( J:247647 )

♂ • after 22 weeks on a HFD, a trend towards an increased number of fat cells is observed in epididymal WAT but not in stromal WAT

decreased white fat cell size ( J:247647 )

♂ • after 6 and 22 weeks on an HFD, adipocyte size is significantly reduced in epididymal WAT relative to that in wild-type controls; a trend towards smaller adipocytes is also observed stromal WAT

decreased white adipose tissue mass ( J:247647 )

♂ • after 22 weeks on a HFD, wet weight of stromal WAT is significantly lower than that in wild-type controls

♂ • however, wet epididymal WAT weight is normal on a HFD

abnormal white adipose tissue physiology ( J:247647 )

♂ • after BrdU treatment and HFD feeding for 1 week, preadipocyte proliferation is significantly increased in epididymal WAT relative to that in wild-type controls

♂ • after 22 weeks on a HFD, mRNA expression of inflammatory markers Tnf and Il1b in epididymal WAT is significantly lower than that in wild-type controls

homeostasis/metabolism

decreased circulating glucose level ( J:247647 )

♂ • after 12 weeks on a HFD, mice show significantly lower blood glucose levels in the fed ad libitum state than wild-type controls

decreased circulating leptin level ( J:247647 )

♂ • after 12 and 22 weeks on a HFD, plasma leptin levels are significantly lower than those in wild-type controls

increased circulating leptin level ( J:247647 )

♂ • after 5 weeks on a HFD, plasma leptin levels are significantly higher than those in wild-type controls

increased respiratory quotient ( J:247647 )

♂ • after 12 weeks on a HFD, mice show a significantly higher respiratory exchange ratio during the night, indicating enhanced glucose oxidation

♂ • however, energy expenditure and physical activity are normal on a HFD

improved glucose tolerance ( J:247647 )

♂ • after 12 weeks on a HFD, mice show improved glucose tolerance in a GTT relative to wild-type controls

♂ • however, glucose tolerance is normal on a regular chow diet

increased insulin sensitivity ( J:247647 )

♂ • after 8 weeks on a HFD, mice show increased insulin sensitivity in an ITT relative to wild-type controls

♂ • however, insulin tolerance is normal on a regular chow diet

decreased collagen level ( J:247647 )

♂ • after 22 weeks on a HFD, total tissue collagen content and Trichrome staining for collagen in epididymal WAT is significantly less than that in wild-type controls

behavior/neurological

increased food intake ( J:247647 )

♂ • after 10 weeks on a HFD, food intake is significantly increased during the dark phase relative to that in wild-type controls

cellular

abnormal extracellular matrix morphology ( J:247647 )

♂ • on a HFD, mRNA expression of Loxl1 (lysyl oxidase-like 1) and Loxl3 (lysyl oxidase-like 3) and protein expression of LOXL3 in epididymal WAT is significantly lower than that in HFD-fed wild-type controls, suggesting altered adipose extracellular matrix remodeling/composition

growth/size/body

growth/size/body region phenotype ( J:247647 )

♂N • mice show no significant changes in weight gain and body composition relative to wild-type controls whether on a normal chow or a high-fat diet

liver/biliary system

liver/biliary system phenotype ( J:247647 )

♂N • on a HFD, mice show no significant changes in wet liver weight or liver triglyceride (TG) content relative to wild-type controls

skeleton

skeleton phenotype ( J:247647 )

♂N • on a HFD, mice show no significant differences in femur length relative to wild-type controls