Phenotypes associated with this allele

• Allele Symbol: Lpcat2^tm1.1Tksh
• Allele Name: targeted mutation 1.1, Takao Shimizu
• Allele ID: MGI:6343500
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Lpcat2^tm1.1Tksh/Lpcat2^tm1.1Tksh	B6N(Cg)-Lpcat2^tm1.1Tksh	MGI:7867324

Genotype
MGI:7867324

hm1

• Allelic Composition: Lpcat2^tm1.1Tksh/Lpcat2^tm1.1Tksh
• Genetic Background: B6N(Cg)-Lpcat2^tm1.1Tksh

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

abnormal phospholipid level ( J:247711 )

• platelet-activating factor (PAF) is almost undetectable in several tissues, including the brain, thymus, liver, spleen, kidney, colon, and spinal cord under steady-state conditions

• however, other phosphatidylcholine (PC) compositions are largely unaltered in these tissues

abnormal enzyme/coenzyme activity ( J:247711 )

• no PAF biosynthetic activity (lyso-PAF acetyltransferase activity) is observed in peritoneal macrophages after stimulation with LPS for 30 min or 18 h, unlike in similarly stimulated wild-type macrophages

• lyso-PAF acyltransferase activity (PC with arachidonic acid, alkyl-PC biosynthesis) is slightly reduced but not absent in LPS-stimulated peritoneal macrophages, possibly due to the activity of other lysophospholipid acyltransferases

abnormal lipid metabolism ( J:247711 )

• no platelet-activating factor (PAF) production is detected in peritoneal macrophages after stimulation with the calcium ionophore A23187 or in several tissues, including the spinal cord, under steady-state conditions

• however, no changes in the abundance of other phosphatidylcholines (PCs) are noted in A23187-stimulated macrophages or in spinal cord, brain, thymus, liver, spleen, kidney, and colon tissues

behavior/neurological

hyporesponsive to tactile stimuli ( J:247711 )

♂ • at 2 weeks after partial sciatic ligation (PSL), the resultant pain level (allodynia score) in male mice is significantly lower than in wild-type controls, as assessed by stroking the injured leg with a paintbrush

increased mechanical nociceptive threshold ( J:247711 )

♂ • after partial sciatic ligation (PSL), mechanical allodynia is largely attenuated in the von Frey filament assay, with male mice showing a significantly higher paw withdrawal threshold than wild-type controls