Phenotypes associated with this allele

• Allele Symbol: Orc1^tm1.2Gle
• Allele Name: targeted mutation 1.2, Gustavo Leone
• Allele ID: MGI:6355394
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Orc1^tm1.2Gle/Orc1^tm1.2Gle	involves: C57BL/6 * SJL	MGI:7408227
cn2	Orc1^tm1.1Gle/Orc1^tm1.2Gle Edil3^Tg(Sox2-cre)1Amc/Edil3^+	involves: C57BL/6 * CBA * SJL	MGI:7408228
cn3	Orc1^tm1.1Gle/Orc1^tm1.2Gle Speer6-ps1^Tg(Alb-cre)21Mgn/Speer6-ps1^+	involves: C57BL/6 * DBA * SJL	MGI:7408239
cn4	Orc1^tm1.1Gle/Orc1^tm1.2Gle Prl2c2^tm1(cre)Gle/Prl2c2^+	involves: C57BL/6 * SJL	MGI:7408236

Genotype
MGI:7408227

hm1

• Allelic Composition: Orc1^tm1.2Gle/Orc1^tm1.2Gle
• Genetic Background: involves: C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality at implantation, complete penetrance ( J:272584 )

• although E3.5 blastocysts are recovered at the expected frequency, no viable embryos are found beyond E6.5 indicating peri-implantation lethality

Genotype
MGI:7408228

cn2

• Allelic Composition: Orc1^tm1.1Gle/Orc1^tm1.2Gle; Edil3^{Tg(Sox2-cre)1Amc}/Edil3⁺
• Genetic Background: involves: C57BL/6 * CBA * SJL

mortality/aging

embryonic lethality, complete penetrance ( J:272584 )

• no live mice are recovered at E10.5 or at 2 weeks of age

Genotype
MGI:7408239

cn3

• Allelic Composition: Orc1^tm1.1Gle/Orc1^tm1.2Gle; Speer6-ps1^{Tg(Alb-cre)21Mgn}/Speer6-ps1⁺
• Genetic Background: involves: C57BL/6 * DBA * SJL

liver/biliary system

increased hepatocyte karyomegaly ( J:272584 )

• at 3 weeks of age, hepatocytes are strikingly larger with larger nuclei than controls

decreased hepatocyte number ( J:272584 )

• fewer hepatocytes are observed relative to controls

• however, liver gross morphology is normal from 3 weeks to 3 months and liver to body weight ratios remain normal up to 6 months of age

cellular

polyploidy ( J:272584 )

• at 3 weeks of age, an increased proportion of hepatocytes with 4C DNA content is observed

• by 3 months of age, a larger proportion of hepatocytes with 8C or greater DNA content is observed

increased hepatocyte karyomegaly ( J:272584 )

• at 3 weeks of age, hepatocytes are strikingly larger with larger nuclei than controls

abnormal DNA replication ( J:272584 )

• hepatocytes endoreduplicate their genomic DNA prematurely

homeostasis/metabolism

abnormal DNA replication ( J:272584 )

• hepatocytes endoreduplicate their genomic DNA prematurely

Genotype
MGI:7408236

cn4

• Allelic Composition: Orc1^tm1.1Gle/Orc1^tm1.2Gle; Prl2c2^tm1(cre)Gle/Prl2c2⁺
• Genetic Background: involves: C57BL/6 * SJL

normal phenotype

no abnormal phenotype detected ( J:272584 )

• placental development is normal, embryos are carried to term and all pups born exhibit no apparent abnormalities