Phenotypes associated with this allele

• Allele Symbol: Cnpy2^tm1.2Zhli
• Allele Name: targeted mutation 1.2 Zihai Li
• Allele ID: MGI:6355915
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cnpy2^tm1.2Zhli/Cnpy2^tm1.2Zhli	B6.Cg-Cnpy2^tm1.2Zhli	MGI:6358735

Genotype
MGI:6358735

hm1

• Allelic Composition: Cnpy2^tm1.2Zhli/Cnpy2^tm1.2Zhli
• Genetic Background: B6.Cg-Cnpy2^tm1.2Zhli

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

liver/biliary system

liver/biliary system phenotype ( J:277868 )

N • under specific pathogen-free conditions, homozygotes survive beyond 16 months of age with no alterations in liver histology or baseline liver function

decreased susceptibility to hepatic steatosis ( J:277868 )

• at 24 h post tunicamycin treatment, mice fail to exhibit any significant hepatosteatosis, unlike similarly treated wild-type and heterozygous controls

decreased susceptibility to diet-induced hepatic steatosis ( J:277868 )

• when fed a high fat diet for 10 weeks, mice exhibit less pale and buoyant livers with significantly decreased accumulation of lipid droplets in hepatocytes relative to similarly fed heterozygous controls

cellular

decreased sensitivity to induced cell death ( J:277868 )

• following treatment with UPR inducers (tunicamycin or thapsigargin), isolated hepatocytes exhibit significantly less endoplasmic reticulum (ER) stress-induced death than similarly treated wild-type or heterozygous hepatocytes, as determined by a cell viability assay

decreased endoplasmic reticulum stress ( J:277868 )

• at 24 h post treatment with the unfolded protein response (UPR) inducer tunicamycin, no CHOP induction is observed in liver and kidney tissues, unlike in wild-type and heterozygous controls

• at 16 h post treatment with tunicamycin or thapsigargin, isolated hepatocytes exhibit significantly reduced levels of ER stress and UPR-associated proteins relative to similarly treated wild-type and heterozygous hepatocytes

• when fed a high fat diet for 10 weeks, mice show a severe reduction of UPR marker induction in liver lysates relative to similarly fed heterozygous controls

growth/size/body

decreased susceptibility to diet-induced obesity ( J:277868 )

• when fed a high fat diet for 10 weeks, mice gain significantly less body weight than similarly fed heterozygous controls

decreased body size ( J:277868 )

• at baseline, homozygotes are slightly but consistently smaller than wild-type or heterozygous controls

• however, no developmental defects are observed

homeostasis/metabolism

decreased susceptibility to diet-induced obesity ( J:277868 )

• when fed a high fat diet for 10 weeks, mice gain significantly less body weight than similarly fed heterozygous controls

decreased circulating triglyceride level ( J:277868 )

• when fed a high fat diet for 10 weeks, mice exhibit significantly lower serum triglyceride levels than similarly fed heterozygous controls

decreased circulating alanine transaminase level ( J:277868 )

• at 24 h post tunicamycin treatment, mice exhibit significantly lower serum alanine aminotransferase (ALT) levels than similarly treated heterozygous controls

• when fed a high fat diet for 10 weeks, mice exhibit significantly lower serum ALT levels than similarly fed heterozygous controls

decreased physiological sensitivity to xenobiotic ( J:277868 )

• at 24 h post tunicamycin treatment, mice fail to exhibit any significant weight loss, liver damage or hepatosteatosis and show a smaller increase in serum alanine aminotransferase (ALT) levels relative to similarly treated heterozygous controls