Phenotypes associated with this allele

• Allele Symbol: Rubcnl^em1Qsun
• Allele Name: endonuclease-mediated mutation 1, Qiming Sun
• Allele ID: MGI:6356294
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Rubcnl^em1Qsun/Rubcnl^em1Qsun Speer6-ps1^Tg(Alb-cre)21Mgn/Speer6-ps1^+	B6.Cg-Speer6-ps1^Tg(Alb-cre)21Mgn Rubcnl^em1Qsun	MGI:6361078

Genotype
MGI:6361078

cn1

• Allelic Composition: Rubcnl^em1Qsun/Rubcnl^em1Qsun; Speer6-ps1^{Tg(Alb-cre)21Mgn}/Speer6-ps1⁺
• Genetic Background: B6.Cg-Speer6-ps1^{Tg(Alb-cre)21Mgn} Rubcnl^em1Qsun

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

liver/biliary system

increased liver weight ( J:272052 )

• liver weight to body weight ratio is significantly increased at 12 weeks of age

• however, body weight is normal

increased liver glycogen level ( J:272052 )

• hepatic glycogen levels are significantly increased at 8 weeks of age

increased liver triglyceride level ( J:272052 )

• hepatic triglyceride levels are significantly increased at 12 weeks of age

hepatic steatosis ( J:272052 )

• Oil red O staining revealed lipid accumulation in liver tissues at 8 weeks of age

liver fibrosis ( J:272052 )

• collagen deposition is significantly increased at 8 weeks of age, suggesting liver fibrosis

• however, no severe hepatomegaly or tumorigenesis are observed over a period of 4 months after birth

abnormal liver physiology ( J:272052 )

• after 24 h of fasting, liver tissue ketone body levels are significantly lower than those in wild-type controls

• however, liver tissue ketone body levels are normal under fed conditions

homeostasis/metabolism

impaired autophagy ( J:272052 )

• protein levels of the autophagy markers p62 and LC3 are increased in the liver at 8 weeks of age, indicating impaired autophagosome maturation

• TEM analysis revealed a significantly increased number of autophagosomes surrounding lipid droplets in hepatocytes at 12 weeks of age

decreased circulating ketone body level ( J:272052 )

• after 24 h of fasting, total serum ketone body levels are significantly lower than those in wild-type controls

• however, total serum ketone body levels are normal under fed conditions

increased liver glycogen level ( J:272052 )

• hepatic glycogen levels are significantly increased at 8 weeks of age

increased liver triglyceride level ( J:272052 )

• hepatic triglyceride levels are significantly increased at 12 weeks of age

cellular

impaired autophagy ( J:272052 )

• protein levels of the autophagy markers p62 and LC3 are increased in the liver at 8 weeks of age, indicating impaired autophagosome maturation

• TEM analysis revealed a significantly increased number of autophagosomes surrounding lipid droplets in hepatocytes at 12 weeks of age

growth/size/body

increased liver weight ( J:272052 )

• liver weight to body weight ratio is significantly increased at 12 weeks of age

• however, body weight is normal