Analysis Tools|
Allele Symbol Allele Name Allele ID |
Stra8em1(GFP/cre)Smoc endonuclease-mediated mutation 1, Shanghai Model Organisms Center MGI:6356355 |
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| Summary |
6 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
 N |
• male mice are fertile and exhibit normal spermatogenesis with no detectable defects in meiosis or spermiogenesis
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• N6-methyladenosine (m6A) levels are reduced by 55-65% in pachytene spermatocytes and by 45% in round spermatids relative to controls
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• caudal epididymal sperm count is only ~2% of that in control mice
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• >80% of caudal epididymal sperm exhibit abnormal heads
• elongated spermatids show abnormal head morphologies at epithelial stages I-VIII
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• elongated spermatids show abnormal head morphologies
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• severely decreased from step 13 to step 16 at epithelial stages I-VIII
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• progressive motility of caudal epididymal sperm is severely reduced at 2 months of age
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• CASA analysis revealed that total motility of caudal epididymal sperm is severely reduced at 2 months of age, indicating defects in sperm flagella
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• seminiferous tubules contain very few mature spermatozoa
• however, no detectable abnormalities are observed in germ cells up to step 12 of elongating spermatids
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• adult testes are significantly smaller than those in controls
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• adult testis/body weight (%) is significantly lower than that in controls
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• spermatid differentiation is blocked in late stages of spermiogenesis
• unexpectedly, male meiosis is normal
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• caudal epididymal sperm count is only ~2% of that in control mice
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• >80% of caudal epididymal sperm exhibit abnormal heads
• elongated spermatids show abnormal head morphologies at epithelial stages I-VIII
|
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• elongated spermatids show abnormal head morphologies
|
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• severely decreased from step 13 to step 16 at epithelial stages I-VIII
|
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• progressive motility of caudal epididymal sperm is severely reduced at 2 months of age
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• CASA analysis revealed that total motility of caudal epididymal sperm is severely reduced at 2 months of age, indicating defects in sperm flagella
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• N6-methyladenosine (m6A) levels in spermatids of double knockout mice are significantly lower than those in single Mettl3em1Chhe or Mettl14em1Chhe knockouts
• however, no significant differences in m6A levels are noted between double knockout and single knockout spermatocytes
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• spermatids from double mutant mice show impaired translation of haploid-specific genes that are essential for spermiogenesis
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• spermatids from double mutant mice show impaired translation of haploid-specific genes that are essential for spermiogenesis
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• seminiferous tubules contain very few mature spermatozoa
• however, no detectable abnormalities are observed in germ cells up to step 12 of elongating spermatids
|
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• adult testes are significantly smaller than those in controls
|
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• adult testis/body weight (%) is significantly lower than that in controls
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
N |
• male mice are fertile and exhibit normal spermatogenesis with no detectable defects in meiosis or spermiogenesis
|
|
|
• N6-methyladenosine (m6A) levels are reduced by 55-65% in pachytene spermatocytes and by 45% in round spermatids relative to controls
|
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
 |
• fewer oocytes are detected at P1 and P3
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• no oocytes are observed at P10, suggesting premature ovarian failure
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• no round or elongated spermatids are observed in the seminiferous tubules at P42
• ratios of WT1+ Sertoli cells to PLZF+ cells (undifferentiated and differentiating spermatogonia) are similar to those in wild-type testes
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• at P42, epididymides are consistently devoid of mature spermatozoa
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• incomplete synapsis is observed in testes sections stained for SYCP1 and SYCP3 and in spermatocytes stained for HORMAD1 (an unsynapsed chromosome marker) and SYCP3
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• at P1, meiotic primordial germ cells (PGCs) show defects in RAD51 and DMC1 removal; PGCs are RAD51- and DMC1-positive throughout the nucleus, whereas wild-type PGCs are at diplotene stage with no or weak RAD51/DMC1 signals
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• persistent foci of RAD51 and DMC1 foci (markers of early recombination intermediates in meiosis) are found in spermatocytes at the zygotene-like stage; extensive RAD51 and DMC1 foci are detected on both unsynapsed and synapsed regions
• number of RAD51 foci only shows a 13% decrease while number of DMC1 foci exhibits just a 23% decrease during the early-zygotene to zygotene-like transition, indicating defects in RAD51/DMC1 removal during meiotic recombination
• at P42, intensity of RAD51 and DMC1 signals is stronger than in wild-type testes
• RAD51 and DMC1 foci frequently co-localize with TEX11 and SHOC1; in zygotene-like spermatocytes, co-localization frequencies for RAD51/TEX11 and DMC1/SHOC1 pairs are 42% and 37%, respectively
• fewer MSH4 foci are observed in zygotene-like spermatocytes; 87% of MSH4 foci co-localize with DMC1, while MLH1 foci (a marker of late recombination intermediates) are almost absent, suggesting that defective RAD51/DMC1 removal impairs meiotic homologous recombination
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• even in the most advanced zygonema-like spermatocytes, SYCP1 stretches do not extend to the full length, remaining short and sometimes increasing in numbers
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• at P42, testes show a massive number of TUNEL+ cells
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• ovaries are significantly smaller than in wild-type controls
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• after P25, ovaries are almost undetectable and hard to be dissected from the oviducts
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• at P42, seminiferous tubules are smaller with large cavities and no round or elongated spermatids are observed
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• at P42, testes are significantly smaller than in wild-type controls
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• at P42, testis weight is 21.05 +/- 0.80 mg versus 72.48 +/- 3.01 mg in wild-type controls
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• spermatogenesis is arrested at meiotic prophase I
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• spermatocytes show impaired meiotic prophase I progression; at P21, pachynema and diplonema with gammaH2AX signals (marking double strand breaks) on sex bodies are not found, unlike in wild-type testes
• ~60% of spermatocytes are arrested at a zygotene-like stage that is similar to the late-zygonema in wild-type testes
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• both male and female mice are infertile
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• fewer oocytes are detected at P1 and P3
|
|
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• no oocytes are observed at P10, suggesting premature ovarian failure
|
|
|
• no round or elongated spermatids are observed in the seminiferous tubules at P42
• ratios of WT1+ Sertoli cells to PLZF+ cells (undifferentiated and differentiating spermatogonia) are similar to those in wild-type testes
|
|
|
• at P42, epididymides are consistently devoid of mature spermatozoa
|
|
|
• incomplete synapsis is observed in testes sections stained for SYCP1 and SYCP3 and in spermatocytes stained for HORMAD1 (an unsynapsed chromosome marker) and SYCP3
|
|
|
• at P1, meiotic primordial germ cells (PGCs) show defects in RAD51 and DMC1 removal; PGCs are RAD51- and DMC1-positive throughout the nucleus, whereas wild-type PGCs are at diplotene stage with no or weak RAD51/DMC1 signals
|
|
|
• persistent foci of RAD51 and DMC1 foci (markers of early recombination intermediates in meiosis) are found in spermatocytes at the zygotene-like stage; extensive RAD51 and DMC1 foci are detected on both unsynapsed and synapsed regions
• number of RAD51 foci only shows a 13% decrease while number of DMC1 foci exhibits just a 23% decrease during the early-zygotene to zygotene-like transition, indicating defects in RAD51/DMC1 removal during meiotic recombination
• at P42, intensity of RAD51 and DMC1 signals is stronger than in wild-type testes
• RAD51 and DMC1 foci frequently co-localize with TEX11 and SHOC1; in zygotene-like spermatocytes, co-localization frequencies for RAD51/TEX11 and DMC1/SHOC1 pairs are 42% and 37%, respectively
• fewer MSH4 foci are observed in zygotene-like spermatocytes; 87% of MSH4 foci co-localize with DMC1, while MLH1 foci (a marker of late recombination intermediates) are almost absent, suggesting that defective RAD51/DMC1 removal impairs meiotic homologous recombination
|
|
|
• spermatocytes show impaired meiotic prophase I progression; at P21, pachynema and diplonema with gammaH2AX signals (marking double strand breaks) on sex bodies are not found, unlike in wild-type testes
• ~60% of spermatocytes are arrested at a zygotene-like stage that is similar to the late-zygonema in wild-type testes
|
|
|
• even in the most advanced zygonema-like spermatocytes, SYCP1 stretches do not extend to the full length, remaining short and sometimes increasing in numbers
|
|
|
• at P42, testes show a massive number of TUNEL+ cells
|
|
|
• ovaries are significantly smaller than in wild-type controls
|
|
|
• after P25, ovaries are almost undetectable and hard to be dissected from the oviducts
|
|
|
• at P42, seminiferous tubules are smaller with large cavities and no round or elongated spermatids are observed
|
|
|
• at P42, testes are significantly smaller than in wild-type controls
|
|
|
• at P42, testis weight is 21.05 +/- 0.80 mg versus 72.48 +/- 3.01 mg in wild-type controls
|
|
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
N |
• adult male mice show normal gross testis morphology and testis/body weight ratio relative to wild-type controls
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|
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• adult males exhibit a significantly lower cauda epididymal sperm count than wild-type controls
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• about 50% of cauda epididymal sperm are deformed with various structural anomalies
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• adult testes show a significant increase in TUNEL+ apoptotic sperm
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• sperm progressive motility is markedly impaired
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• sperm motility is markedly impaired
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• cauda epididymal sperm from adult males fail to fertilize wild-type oocytes in IVF assays
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• adult males exhibit a significantly lower cauda epididymal sperm count than wild-type controls
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• about 50% of cauda epididymal sperm are deformed with various structural anomalies
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• adult testes show a significant increase in TUNEL+ apoptotic sperm
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• sperm progressive motility is markedly impaired
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• sperm motility is markedly impaired
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
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• many seminiferous tubules in P16 and older males are vacuolated due to the absence of spermatocytes and spermatids, as indicated by a lack of XY body formation and absence of PNA signal
• no sperm are seen in adult cauda epididymides
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• germ cell development is arrested in meiotic prophase
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• testes are smaller at P16 and later
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• testes are smaller at P16 and later
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• many seminiferous tubules in P16 and older males are vacuolated due to the absence of spermatocytes and spermatids, as indicated by a lack of XY body formation and absence of PNA signal
• no sperm are seen in adult cauda epididymides
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• germ cell development is arrested in meiotic prophase
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 12/17/2024 MGI 6.24 |
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