Analysis Tools|
Allele Symbol Allele Name Allele ID |
Mettl14em1Chhe endonuclease-mediated mutation 1, Chuan He MGI:6356357 |
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| Summary |
4 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
 N |
• male mice are fertile and exhibit normal spermatogenesis with no detectable defects in meiosis or spermiogenesis
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• N6-methyladenosine (m6A) levels are reduced by 55-65% in pachytene spermatocytes and by 45% in round spermatids relative to controls
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• caudal epididymal sperm count is only ~2% of that in control mice
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• >80% of caudal epididymal sperm exhibit abnormal heads
• elongated spermatids show abnormal head morphologies at epithelial stages I-VIII
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• elongated spermatids show abnormal head morphologies and their number is severely decreased from step 13 to step 16 at epithelial stages I-VIII
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• progressive motility of caudal epididymal sperm is severely reduced at 2 months of age
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• CASA analysis revealed that total motility of caudal epididymal sperm is severely reduced at 2 months of age, indicating defects in sperm flagella
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• seminiferous tubules contain very few mature spermatozoa
• however, no detectable abnormalities are observed in germ cells up to step 12 of elongating spermatids
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• adult testes are significantly smaller than those in controls
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• adult testis/body weight (%) is significantly lower than that in controls
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• spermatid differentiation is blocked in late stages of spermiogenesis
• unexpectedly, male meiosis is normal
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• caudal epididymal sperm count is only ~2% of that in control mice
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• >80% of caudal epididymal sperm exhibit abnormal heads
• elongated spermatids show abnormal head morphologies at epithelial stages I-VIII
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• elongated spermatids show abnormal head morphologies and their number is severely decreased from step 13 to step 16 at epithelial stages I-VIII
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• progressive motility of caudal epididymal sperm is severely reduced at 2 months of age
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• CASA analysis revealed that total motility of caudal epididymal sperm is severely reduced at 2 months of age, indicating defects in sperm flagella
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• N6-methyladenosine (m6A) levels in spermatids of double knockout mice are significantly lower than those in single Mettl3em1Chhe or Mettl14em1Chhe knockouts
• however, no significant differences in m6A levels are noted between double knockout and single knockout spermatocytes
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• spermatids from double mutant mice show impaired translation of haploid-specific genes that are essential for spermiogenesis
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• spermatids from double mutant mice show impaired translation of haploid-specific genes that are essential for spermiogenesis
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• seminiferous tubules contain very few mature spermatozoa
• however, no detectable abnormalities are observed in germ cells up to step 12 of elongating spermatids
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• adult testes are significantly smaller than those in controls
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• adult testis/body weight (%) is significantly lower than that in controls
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• no GFRalpha1+ Asingle (As, early stage undifferentiated spermatogonia) or even PLZF+ undifferentiated spermatogonia are detected at 4 weeks
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• mice exhibit progressive loss of spermatogonial stem cells (SSCs) causing germ cell depletion, similar to Mettl3em1Chhe/Mettl3em1Chhe Tg(Ddx4-cre)1Dcas/0 mice
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• by 6 weeks of age, the seminiferous tubule epithelium is completely devoid of any germ cells, with only SOX9+ Sertoli cells remaining
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• adult testes are smaller than normal
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• no GFRalpha1+ Asingle (As, early stage undifferentiated spermatogonia) or even PLZF+ undifferentiated spermatogonia are detected at 4 weeks
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• mice exhibit progressive loss of spermatogonial stem cells (SSCs) causing germ cell depletion, similar to Mettl3em1Chhe/Mettl3em1Chhe Tg(Ddx4-cre)1Dcas/0 mice
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• N6-methyladenosine (m6A) levels are significantly reduced in THY1+ undifferentiated spermatogonia
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• mRNA m6A depletion dysregulates translation of transcripts that are required for SSC proliferation/differentiation
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• mRNA m6A depletion dysregulates translation of transcripts that are required for SSC proliferation/differentiation
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• by 6 weeks of age, the seminiferous tubule epithelium is completely devoid of any germ cells, with only SOX9+ Sertoli cells remaining
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• adult testes are smaller than normal
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
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• no GFRalpha1+ Asingle (As, early stage undifferentiated spermatogonia) or PLZF+ undifferentiated spermatogonia are detected at 4 weeks
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• double knockout mice exhibit progressive loss of spermatogonial stem cells, similar to single Mettl3em1Chhe or Mettl14em1Chhe knockouts
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• no GFRalpha1+ Asingle (As, early stage undifferentiated spermatogonia) or PLZF+ undifferentiated spermatogonia are detected at 4 weeks
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• double knockout mice exhibit progressive loss of spermatogonial stem cells, similar to single Mettl3em1Chhe or Mettl14em1Chhe knockouts
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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