All Phenotypes Crls1<tm1Geno> MGI Mouse

decreased subcutaneous adipose tissue amount ( J:266083 )

• chow-fed mice show a significant reduction in subcutaneous white adipose tissue (scWAT) weight, and this phenotype is exacerbated on a HFD

abnormal brown adipose tissue morphology ( J:266083 )

• interscapular brown adipose tissue (iBAT) is distinctly paler than that in control mice, due to a virtual ablation of all cardiolipin species

abnormal brown fat cell morphology ( J:266083 )

• brown adipocytes exhibit distorted cellular and lipid droplet morphology in iBAT, as a result of mitochondrial dysfunction

• however, white adipose depots are not severely affected

increased brown fat cell lipid droplet size ( J:266083 )

decreased fat cell mitochondrial DNA content ( J:266083 )

• mtDNA content is markedly decreased in iBAT

• however, mtDNA levels in epididymal white adipose tissue (eWAT) are normal

decreased epididymal fat pad weight ( J:266083 )

• chow-fed mice show a significant reduction in epididymal white adipose tissue (eWAT) weight, and this phenotype is exacerbated on a HFD

decreased interscapular fat pad weight ( J:266083 )

• chow-fed mice show a significant reduction in iBAT weight

decreased adipocyte glucose uptake ( J:266083 )

• following i.p. injection with the beta3 agonist CL-316,243 to specifically stimulate glucose uptake into thermogenic fat, mice exhibit significantly lower glucose uptake into iBAT than controls

abnormal brown adipose tissue physiology ( J:266083 )

• in interscapular brown adipose tissue (iBAT), expression profile of genes linked to thermogenesis, adipogenesis, and mitochondrial respiration more closely resemble that of white fat than brown fat

abnormal brown adipose tissue thermogenesis ( J:266083 )

• mice show a marked reduction in norepinephrine (NE)-induced uncoupled respiration and heat production relative to controls

homeostasis/metabolism phenotype ( J:266083 )

N	• chow-fed mice show no significant alterations in energy expenditure relative to control mice

decreased susceptibility to diet-induced obesity ( J:266083 )

• mice fed a 60% high-fat diet (HFD) gain significantly less weight and have more lean mass but less fat mass than HFD-fed controls

impaired adaptive thermogenesis ( J:266083 )

• mice show a significantly greater drop in core body temperature than controls in response to acute cold exposure (a 22 to 4 degrees Celsius challenge for 1.5 h), indicating reduced cold tolerance

• cold exposure fails to induce Ucp1 transcription in iBAT or subcutaneous white adipose tissue (scWAT), unlike in control mice

increased fasting circulating glucose level ( J:266083 )

• mice fed a HFD show increased fasting blood glucose levels

decreased oxygen consumption ( J:266083 )

• mice show a marked reduction in norepinephrine (NE)-induced uncoupled respiration relative to controls

decreased respiratory quotient ( J:266083 )

• mice housed in metabolic cages show a marked reduction in the daily respiratory quotient (RQ) biorhythm relative to controls, indicating decreased metabolic flexibility

insulin resistance ( J:266083 )

• chow-fed mice show decreased insulin sensitivity relative to control mice

• mice fed a 60% high-fat diet (HFD) are completely refractory to insulin

decreased fat cell mitochondrial DNA content ( J:266083 )

• mtDNA content is markedly decreased in iBAT

• however, mtDNA levels in epididymal white adipose tissue (eWAT) are normal

decreased adipocyte glucose uptake ( J:266083 )

• following i.p. injection with the beta3 agonist CL-316,243 to specifically stimulate glucose uptake into thermogenic fat, mice exhibit significantly lower glucose uptake into iBAT than controls

disorganized mitochondrial cristae ( J:266083 )

• mitochondria isolated from iBAT show disrupted cristae structure

decreased mitochondrial size ( J:266083 )

• mitochondria isolated from iBAT show reduced mitochondrial mass (as measured by citrate synthase activity)

abnormal respiratory electron transport chain ( J:266083 )

• mitochondria isolated from iBAT show altered respiratory chain complex and super complex formation and reduced GDP-inhibitable (UCP1-linked) respiration

growth/size/body region phenotype ( J:266083 )

N	• chow-fed mice show no significant alterations in body weight or composition relative to control mice

decreased susceptibility to diet-induced obesity ( J:266083 )

• mice fed a 60% high-fat diet (HFD) gain significantly less weight and have more lean mass but less fat mass than HFD-fed controls

increased liver weight ( J:266083 )

• chow-fed mice show increased liver weight relative to control mice, and this phenotype is exacerbated on a HFD

• however, liver triglyceride levels are normal

increased liver weight ( J:266083 )

• chow-fed mice show increased liver weight relative to control mice, and this phenotype is exacerbated on a HFD

• however, liver triglyceride levels are normal

decreased subcutaneous adipose tissue amount ( J:266083 )

• chow-fed mice show a significant reduction in subcutaneous white adipose tissue (scWAT) weight, and this phenotype is exacerbated on a HFD

behavior/neurological phenotype ( J:266083 )

N	• chow-fed mice show no significant alterations in food intake or physical activity relative to control mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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