Phenotypes associated with this allele

• Allele Symbol: Zfp131^tm1.1Mytk
• Allele Name: targeted mutation 1.1, Shoichiro Miyatake
• Allele ID: MGI:6356577
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Zfp131^tm1.1Mytk/Zfp131^tm1.1Mytk Tg(Cd4-cre)1Cwi/0	involves: C57BL/6 * C57BL/6N * DBA/2	MGI:6765907
cn2	Zfp131^tm1.1Mytk/Zfp131^tm1.2Mytk Tg(Lck-cre)1Jtak/0	involves: C57BL/6N * FVB/N	MGI:6765906

Genotype
MGI:6765907

cn1

• Allelic Composition: Zfp131^tm1.1Mytk/Zfp131^tm1.1Mytk; Tg(Cd4-cre)1Cwi/0
• Genetic Background: involves: C57BL/6 * C57BL/6N * DBA/2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:271921 )

N • differentiation and maturation of CD4 single-positive (SP) and CD8 SP populations within the thymus are normal, with no significant alterations in the numbers of total, double-negative (DN), double-positive (DP), CD4 SP, and CD8 SP thymocytes

decreased T cell proliferation ( J:271921 )

• after in vitro stimulation with anti-CD3epsilon and anti-CD28 mAbs, increase in CD4 SP thymocytes cell number is significantly lower than in control mice at days 3 and 5 post-stimulation

• after stimulation with anti-CD3epsilon and anti-CD28 mAbs for 4 h, CD4 SP thymocytes show higher mRNA expression of cell-cycle regulator Cdkn1a (p21^Cip1) than control cells

• proliferation defect is not rescued in the presence of exogenous IL-2

abnormal thymocyte activation ( J:271921 )

• after in vitro stimulation with anti-CD3epsilon and anti-CD28 mAbs, CD4 SP thymocytes show significantly lower mRNA expression of genes involved in effector cell induction (Il2 and Tbx2)

abnormal T cell subpopulation ratio ( J:271921 )

• increased ratio of memory-phenotype versus naive T cells of either CD4 or CD8 cells in periphery

• many peripheral memory-phenotype T cells retain non-deleted floxed allele suggesting that Znf131-deficient naive T cells released from the thymus are unable to expand to maintain T cell homeostasis in periphery

decreased T cell number ( J:271921 )

• despite normal thymic selection and T cell maturation in the thymus, mice show a significant reduction in absolute numbers of CD3+, CD4+ and CD8+ cells in spleen

• naive T cells released from the thymus are not able to proliferate to maintain naive T cell pool in periphery

decreased CD4-positive, alpha-beta T cell number ( J:271921 )

• significant reduction in absolute numbers of CD4+ cells in spleen

decreased CD8-positive, alpha-beta T cell number ( J:271921 )

• significant reduction in absolute numbers of CD8+ cells in spleen

hematopoietic system

decreased T cell proliferation ( J:271921 )

• after in vitro stimulation with anti-CD3epsilon and anti-CD28 mAbs, increase in CD4 SP thymocytes cell number is significantly lower than in control mice at days 3 and 5 post-stimulation

• after stimulation with anti-CD3epsilon and anti-CD28 mAbs for 4 h, CD4 SP thymocytes show higher mRNA expression of cell-cycle regulator Cdkn1a (p21^Cip1) than control cells

• proliferation defect is not rescued in the presence of exogenous IL-2

abnormal thymocyte activation ( J:271921 )

• after in vitro stimulation with anti-CD3epsilon and anti-CD28 mAbs, CD4 SP thymocytes show significantly lower mRNA expression of genes involved in effector cell induction (Il2 and Tbx2)

abnormal T cell subpopulation ratio ( J:271921 )

• increased ratio of memory-phenotype versus naive T cells of either CD4 or CD8 cells in periphery

• many peripheral memory-phenotype T cells retain non-deleted floxed allele suggesting that Znf131-deficient naive T cells released from the thymus are unable to expand to maintain T cell homeostasis in periphery

decreased T cell number ( J:271921 )

• despite normal thymic selection and T cell maturation in the thymus, mice show a significant reduction in absolute numbers of CD3+, CD4+ and CD8+ cells in spleen

• naive T cells released from the thymus are not able to proliferate to maintain naive T cell pool in periphery

decreased CD4-positive, alpha-beta T cell number ( J:271921 )

• significant reduction in absolute numbers of CD4+ cells in spleen

decreased CD8-positive, alpha-beta T cell number ( J:271921 )

• significant reduction in absolute numbers of CD8+ cells in spleen

endocrine/exocrine glands

abnormal thymocyte activation ( J:271921 )

• after in vitro stimulation with anti-CD3epsilon and anti-CD28 mAbs, CD4 SP thymocytes show significantly lower mRNA expression of genes involved in effector cell induction (Il2 and Tbx2)

cellular

decreased T cell proliferation ( J:271921 )

• after in vitro stimulation with anti-CD3epsilon and anti-CD28 mAbs, increase in CD4 SP thymocytes cell number is significantly lower than in control mice at days 3 and 5 post-stimulation

• after stimulation with anti-CD3epsilon and anti-CD28 mAbs for 4 h, CD4 SP thymocytes show higher mRNA expression of cell-cycle regulator Cdkn1a (p21^Cip1) than control cells

• proliferation defect is not rescued in the presence of exogenous IL-2

Genotype
MGI:6765906

cn2

• Allelic Composition: Zfp131^tm1.1Mytk/Zfp131^tm1.2Mytk; Tg(Lck-cre)1Jtak/0
• Genetic Background: involves: C57BL/6N * FVB/N

immune system

decreased T cell proliferation ( J:271921 )

• BrdU incorporation of double-negative (DN) T cells is 15.1% versus 26.6% in controls, indicating that cell proliferation accompanied with DN-to-DP transition after beta-selection is suppressed

• Cdkn1a (p21^Cip1) mRNA expression is increased in DN3 (Lin-CD25+CD117-) populations

• however, expression of Myc (c-Myc) mRNA and protein in DN3 is normal

decreased thymocyte number ( J:271921 )

• early T-cell precursor (ETP) numbers are significantly reduced

• total thymocyte number is reduced by ~25-fold

abnormal T cell differentiation ( J:271921 )

• abnormal differentiation from DN3a to DN3b and DN4 compartments

• however, DNA rearrangement of the TCRbeta locus is normal

abnormal double-negative T cell morphology ( J:271921 )

• % of intracellular TCRbeta (icTCRb)+ cells in DN4 is reduced

• however, % of icTCRb+ cells in DN3 is normal

decreased double-negative T cell number ( J:271921 )

decreased DN2 thymocyte number ( J:271921 )

decreased DN3 thymocyte number ( J:271921 )

• DN3 thymocyte number is reduced by >2.5-fold

• % of DN3b cells (defined by CD27^hi and increased cell size) in DN3 population is decreased while upregulation of CD27 expression is reduced, suggesting defects in pre-TCR signaling

• however, % of intracellular TCRbeta (icTCRb)+ cells in DN3 is normal

decreased DN4 thymocyte number ( J:271921 )

• DN4 thymocyte number is reduced by 20-fold

• % of intracellular TCRbeta (icTCRb)+ cells in DN4 is reduced

decreased double-positive T cell number ( J:271921 )

arrested T cell differentiation ( J:271921 )

• severe block in the transition from the double-negative DN3/4 stage to the double-positive (DP) stage

• most cells accumulate at the DN3 (CD25+CD117-) stage where beta-selection occurs

• however, Notch signaling is not impaired at the DN3 stage and gamma-delta T cell development is normal

decreased CD4-positive, alpha-beta T cell number ( J:271921 )

• significant decrease in CD4 single-positive (SP) population

decreased CD8-positive, alpha-beta T cell number ( J:271921 )

• significant decrease in CD8 immature single-positive (SP) population

hematopoietic system

decreased T cell proliferation ( J:271921 )

• BrdU incorporation of double-negative (DN) T cells is 15.1% versus 26.6% in controls, indicating that cell proliferation accompanied with DN-to-DP transition after beta-selection is suppressed

• Cdkn1a (p21^Cip1) mRNA expression is increased in DN3 (Lin-CD25+CD117-) populations

• however, expression of Myc (c-Myc) mRNA and protein in DN3 is normal

decreased thymocyte number ( J:271921 )

• early T-cell precursor (ETP) numbers are significantly reduced

• total thymocyte number is reduced by ~25-fold

abnormal T cell differentiation ( J:271921 )

• abnormal differentiation from DN3a to DN3b and DN4 compartments

• however, DNA rearrangement of the TCRbeta locus is normal

abnormal double-negative T cell morphology ( J:271921 )

• % of intracellular TCRbeta (icTCRb)+ cells in DN4 is reduced

• however, % of icTCRb+ cells in DN3 is normal

decreased double-negative T cell number ( J:271921 )

decreased DN2 thymocyte number ( J:271921 )

decreased DN3 thymocyte number ( J:271921 )

• DN3 thymocyte number is reduced by >2.5-fold

• % of DN3b cells (defined by CD27^hi and increased cell size) in DN3 population is decreased while upregulation of CD27 expression is reduced, suggesting defects in pre-TCR signaling

• however, % of intracellular TCRbeta (icTCRb)+ cells in DN3 is normal

decreased DN4 thymocyte number ( J:271921 )

• DN4 thymocyte number is reduced by 20-fold

• % of intracellular TCRbeta (icTCRb)+ cells in DN4 is reduced

decreased double-positive T cell number ( J:271921 )

arrested T cell differentiation ( J:271921 )

• severe block in the transition from the double-negative DN3/4 stage to the double-positive (DP) stage

• most cells accumulate at the DN3 (CD25+CD117-) stage where beta-selection occurs

• however, Notch signaling is not impaired at the DN3 stage and gamma-delta T cell development is normal

decreased CD4-positive, alpha-beta T cell number ( J:271921 )

• significant decrease in CD4 single-positive (SP) population

decreased CD8-positive, alpha-beta T cell number ( J:271921 )

• significant decrease in CD8 immature single-positive (SP) population

endocrine/exocrine glands

decreased thymocyte number ( J:271921 )

• early T-cell precursor (ETP) numbers are significantly reduced

• total thymocyte number is reduced by ~25-fold

decreased DN2 thymocyte number ( J:271921 )

decreased DN3 thymocyte number ( J:271921 )

• DN3 thymocyte number is reduced by >2.5-fold

• % of DN3b cells (defined by CD27^hi and increased cell size) in DN3 population is decreased while upregulation of CD27 expression is reduced, suggesting defects in pre-TCR signaling

• however, % of intracellular TCRbeta (icTCRb)+ cells in DN3 is normal

decreased DN4 thymocyte number ( J:271921 )

• DN4 thymocyte number is reduced by 20-fold

• % of intracellular TCRbeta (icTCRb)+ cells in DN4 is reduced

cellular

decreased T cell proliferation ( J:271921 )

• BrdU incorporation of double-negative (DN) T cells is 15.1% versus 26.6% in controls, indicating that cell proliferation accompanied with DN-to-DP transition after beta-selection is suppressed

• Cdkn1a (p21^Cip1) mRNA expression is increased in DN3 (Lin-CD25+CD117-) populations

• however, expression of Myc (c-Myc) mRNA and protein in DN3 is normal