Phenotypes associated with this allele

• Allele Symbol: Trim65^em1Cya
• Allele Name: endonuclease-mediated mutation 1, Cyagen Biosciences
• Allele ID: MGI:6356825
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Trim65^em1Cya/Trim65^em1Cya	involves: C57BL/6	MGI:6822325

Genotype
MGI:6822325

hm1

• Allelic Composition: Trim65^em1Cya/Trim65^em1Cya
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

increased susceptibility to Picornaviridae infection induced morbidity/mortality ( J:240099 )

• after i.p. infection with encephalomyocarditis virus (EMCV), all mice die within 3 days post-infection i.e. significantly sooner than similarly infected wild-type controls

immune system

decreased circulating interferon-alpha level ( J:240099 )

• 12 h after i.v. inoculation with EMCV, serum interferon-alpha levels are markedly lower than those in similarly infected wild-type controls

decreased circulating interferon-beta level ( J:240099 )

• 12 h after i.v. inoculation with EMCV, serum interferon-beta levels are markedly lower than those in similarly infected wild-type controls

• in contrast, 12 h after i.v. inoculation with vesicular stomatitis virus (VSV, Indiana strain), serum interferon-beta levels are comparable to those in wild-type controls

decreased interferon-alpha secretion ( J:240099 )

• following infection with EMCV, IFN-alpha secretion by bone marrow derived macrophages (BMDMs) is completely inhibited

• in contrast, following infection with VSV, IFN-alpha secretion by BMDMs is normal

decreased interferon-beta secretion ( J:240099 )

• following EMCV infection, induction of IFN-beta mRNA expression and IFN-beta secretion by BMDMs is completely inhibited

• following stimulation with HMW poly I:C or EMCV-RNA, induction of IFN-beta mRNA expression in BMDMs is abrogated

• in contrast, VSV infection-induced IFN-beta secretion by BMDMs is normal

• IFN-beta mRNA expression in BMDMs is also normal following adenovirus infection, or after stimulation with 5'-triphosphate RNA (3pRNA, an agonist for RIG-I), extracellular LPS (agonist for TLR4), poly I:C (agonist for TLR3) or VSV-RNA

increased susceptibility to Picornaviridae infection ( J:240099 )

• 48 h after i.p. infection with encephalomyocarditis virus (EMCV, strain D), mice exhibit more severe tissue damage and higher virus titer in the pancreas than EMCV-infected wild-type controls

• in contrast, no differences in virus titer are observed in the pancreas 24 h after VSV infection

increased susceptibility to Picornaviridae infection induced morbidity/mortality ( J:240099 )

• after i.p. infection with encephalomyocarditis virus (EMCV), all mice die within 3 days post-infection i.e. significantly sooner than similarly infected wild-type controls

homeostasis/metabolism

decreased circulating interferon-alpha level ( J:240099 )

• 12 h after i.v. inoculation with EMCV, serum interferon-alpha levels are markedly lower than those in similarly infected wild-type controls

decreased circulating interferon-beta level ( J:240099 )

• 12 h after i.v. inoculation with EMCV, serum interferon-beta levels are markedly lower than those in similarly infected wild-type controls

• in contrast, 12 h after i.v. inoculation with vesicular stomatitis virus (VSV, Indiana strain), serum interferon-beta levels are comparable to those in wild-type controls