Phenotypes associated with this allele

• Allele Symbol: Prss55^tm1Zgwg
• Allele Name: targeted mutation 1, Zhugang Wang
• Allele ID: MGI:6357155
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Prss55^tm1Zgwg/Prss55^tm1Zgwg	involves: 129 * C57BL/6	MGI:6358239

Genotype
MGI:6358239

hm1

• Allelic Composition: Prss55^tm1Zgwg/Prss55^tm1Zgwg
• Genetic Background: involves: 129 * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

reproductive system phenotype ( J:278420 )

N • female fertility is normal

N • despite male infertility, no significant alterations in mating activity, sperm production, sperm morphology, sperm motility, acrosome reaction or activity of acrosomal enzymes are observed

impaired sperm migration in female genital tract ( J:278420 )

♂ • in vivo sperm migration in the female genital tract (from uterus into oviduct) is impaired

♂ • less than 10 sperm are present in the oviduct 3.5 h after coitus versus >300 sperm recovered in the oviduct of females mated with wild-type males

♂ • mature ADAM3 is nearly absent from cauda epididyma sperm, while its precursor form expressed in the testis is almost unaffected

male infertility ( J:278420 )

♂ • males are infertile; crossing of male homozygotes with wild-type females does not produce any offspring

♂ • however, the frequency of copulatory plug is normal

decreased fertilization frequency ( J:278420 )

♂ • in vivo fertilization rate of males is dramatically reduced to 4.1% versus 82.9% in wild-type controls

♂ • however, in vitro fertilization rate of sperm is comparable to that of wild-type sperm

impaired sperm-egg recognition ( J:278420 )

• severe defects in sperm-egg recognition in vitro

impaired binding of sperm to zona pellucida ( J:278420 )

♂ • severe defects in sperm binding to either zona-intact or zona-free oocytes in vitro

cellular

impaired sperm migration in female genital tract ( J:278420 )

♂ • in vivo sperm migration in the female genital tract (from uterus into oviduct) is impaired

♂ • less than 10 sperm are present in the oviduct 3.5 h after coitus versus >300 sperm recovered in the oviduct of females mated with wild-type males

♂ • mature ADAM3 is nearly absent from cauda epididyma sperm, while its precursor form expressed in the testis is almost unaffected