Phenotypes associated with this allele

• Allele Symbol: Mecr^{tm1.1(fabI)Akast}
• Allele Name: targeted mutation 1.1, Alexander J Kastaniotis
• Allele ID: MGI:6357890
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mecr^tm1.1(fabI)Akast/Mecr^tm1.1(fabI)Akast	involves: C57BL/6 * C57BL/6JOlaHsd * C57BL/6N * SJL	MGI:6391012

Genotype
MGI:6391012

hm1

• Allelic Composition: Mecr^{tm1.1(fabI)Akast}/Mecr^{tm1.1(fabI)Akast}
• Genetic Background: involves: C57BL/6 * C57BL/6JOlaHsd * C57BL/6N * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:242847 )

• homozygous mutant embryos die between E10.5 and E11.5; all embryos are dead and at varying stages of resorption at E11.5

• no mouse embryonic fibroblasts could be established

embryo

abnormal vitelline vasculature morphology ( J:242847 )

• at E10.5, yolk sac vessels are pale and empty

decreased embryo size ( J:242847 )

• at E10.5, all embryos are smaller than heterozygous or wild-type embryos

abnormal placenta morphology ( J:242847 )

• at E9.5, the fetal placenta is much thinner and disorganized

abnormal trophoblast giant cell morphology ( J:242847 )

• at E9.5, organization of trophoblast giant cells is poor

decreased trophoblast giant cell number ( J:242847 )

• at E9.5, trophoblast giant cell layers are markedly thinner

abnormal spongiotrophoblast layer morphology ( J:242847 )

• at E9.5, organization of spongiotrophoblasts is poor

decreased spongiotrophoblast cell number ( J:242847 )

• at E9.5, spongiotrophoblast layers are markedly thinner

abnormal placenta labyrinth morphology ( J:242847 )

• EM revealed failure of differentiation of labyrinthine trophoblasts as well as maternal and fetal blood spaces in labyrinth at E9.5

abnormal placenta fetal blood space morphology ( J:242847 )

• at E9.5, fetal blood spaces in labyrinth are not evident

abnormal placenta intervillous maternal lacunae morphology ( J:242847 )

• at E9.5, maternal blood spaces are not evident

disorganized placental labyrinth ( J:242847 )

• at E9.5, the labyrinthine layer of the fetal part of placenta is poorly organized

pale yolk sac ( J:242847 )

abnormal placenta development ( J:242847 )

• defects in placental development associated with disturbed peroxisome proliferator-activated receptor signaling

growth/size/body

decreased embryo size ( J:242847 )

• at E10.5, all embryos are smaller than heterozygous or wild-type embryos

cardiovascular system

abnormal vitelline vasculature morphology ( J:242847 )

• at E10.5, yolk sac vessels are pale and empty

delayed heart development ( J:242847 )

• at E10.5, hearts are underdeveloped and resemble embryonic hearts at E8.5-E9.5

• the primitive atrial chamber and ventricle are the only discernible chambers at E10.5; however, trabeculation in myocardial wall of ventricular chambers is visible

• micro-computed tomography revealed that heart and cavity volumes are 19% and 21% smaller, respectively, than in controls and only a two chambered heart is present

abnormal heart size ( J:242847 )

• micro-computed tomography revealed that heart and cavity volumes are 19% and 21% smaller, respectively, than in controls and only a two chambered heart is present at E10.5

abnormal heartbeat ( J:242847 )

• at E10.5, heart beats are weak or absent

absent heartbeat ( J:242847 )

• at E10.5, heart beats are weak or absent

cellular

abnormal mitochondrial morphology ( J:242847 )

• at E9.5, mitochondria in labyrinthine trophoblasts appear clustered, irregular in shape and electron-lucent with no clear cristae structure

abnormal mitochondrial physiology ( J:242847 )

• analysis of several mitochondrial parameters revealed a reduced rate of mitochondrial respiration in permeabilized cells

integument

pallor ( J:242847 )

• at E10.5, all embryos are pale and fragile