Analysis Tools
skeleton
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• decalcified femurs stained with osmium tetroxide show a significantly higher number of adipocytes and fat droplets, indicating increased bone marrow fat accumulation
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• femurs show reduced cortical bone thickness, as shown by micro-CT analysis
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• femurs show decreased bone mineral density at 3, 6 and 12 months of age, as measured by DEXA analysis
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• femurs show reduced trabecular bone volume, as shown by micro-CT analysis
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• in distal femurs, the number of osteocalcin-positive mature osteoblasts per bone surface is significantly lower than that in wild-type controls
• the number and surface of osteoblasts normalized to trabecular bone surface in femur are significantly decreased at 3, 6 and 12 months of age, unlike in age-matched wild-type controls
• however, the number and surface of osteoclasts normalized to trabecular bone surface are unaffected
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• mice show accelerated age-associated bone loss
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• bone formation rate per bone surface is significantly lower than that in wild-type controls, as shown by calcein double-labeling
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adipose tissue
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• decalcified femurs stained with osmium tetroxide show a significantly higher number of adipocytes and fat droplets, indicating increased bone marrow fat accumulation
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limbs/digits/tail
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• femurs show reduced cortical bone thickness, as shown by micro-CT analysis
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