Phenotypes associated with this allele

• Allele Symbol: Cdhr2^{tm1c(EUCOMM)Hmgu}
• Allele Name: targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• Allele ID: MGI:6363535
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Cdhr2^tm1c(EUCOMM)Hmgu/Cdhr2^tm1c(EUCOMM)Hmgu Tg(Vil1-cre)20Syr/0	involves: C57BL/6 * C57BL/6N * DBA/2	MGI:6363536

Genotype
MGI:6363536

cn1

• Allelic Composition: Cdhr2^{tm1c(EUCOMM)Hmgu}/Cdhr2^{tm1c(EUCOMM)Hmgu}; Tg(Vil1-cre)20Syr/0
• Genetic Background: involves: C57BL/6 * C57BL/6N * DBA/2
• Cell Lines: HEPD0749_5_F10

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

decreased body weight ( J:273165 )

• at P90, body weight is significantly lower than that of wild-type controls

postnatal growth retardation ( J:273165 )

• growth begins to lag behind that of wild-type controls in the second month of life into adulthood

digestive/alimentary system

abnormal intestinal epithelium morphology ( J:273165 )

• SEM analysis of the intestinal epithelium revealed bare regions that lack closely spaced villi

• villus surfaces appear rougher and less flat than those in wild-type controls

abnormal enterocyte morphology ( J:273165 )

• enterocytes exhibit a domed apical surface with an abnormal outward/convex curvature

• however, apical-basolateral polarity and simple columnar morphology remain normal

abnormal duodenum crypts of Lieberkuhn morphology ( J:273165 )

• mean crypt depth at the proximal (duodenal) end of the small intestine is significantly decreased

abnormal jejunum morphology ( J:273165 )

• TEM of the brush borders in jejunal tissues revealed that enterocytes show prominent apical doming

abnormal small intestinal microvillus morphology ( J:273165 )

• intermicrovillar adhesion complex (IMAC) components CDHR5, USH1C, and MYO7B are displaced from their normal localization at the tips of microvilli, unlike in wild-type controls

• microvilli are significantly shorter and more variable in length, and show a splayed morphology with significant physical separation and free space between neighboring protrusions

• intermicrovillar adhesion links, normally seen at the tips of wild-type microvilli, are almost entirely absent; in rare cases, remnant link-like structures between some microvilli are observed

• a subset of microvilli show irregular non-cylindrical shapes such that cross-sections show more angular or oblong profiles rather than circular and cross-sectional area is significantly increased

• misshapen microvilli have abnormally large, poorly consolidated core actin bundles or in some cases multiple core-like structures

• microvilli show a striking loss of hexagonal packing order and reduced packing density, with enterocytes having ~33% fewer protrusions per unit apical area relative to wild-type controls

decreased small intestinal villus height ( J:273165 )

• mean villus length at the proximal (duodenal) end of the small intestine is significantly decreased

abnormal enterocyte physiology ( J:273165 )

• levels of key apical enzymes and transporters that are critical for enterocyte function are significantly reduced in the brush border

endocrine/exocrine glands

abnormal duodenum crypts of Lieberkuhn morphology ( J:273165 )

• mean crypt depth at the proximal (duodenal) end of the small intestine is significantly decreased