Phenotypes associated with this allele

• Allele Symbol: Ociad1^tm1.1Msin
• Allele Name: targeted mutation 1.1, Maneesha S Inamdar
• Allele ID: MGI:6367551
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ociad1^tm1.1Msin/Ociad1^tm1.1Msin	involves: BALB/cJ * C57BL/6NCrlj * CBA/JNCrlj	MGI:6367552
ht2	Ociad1^tm1.1Msin/Ociad1^+	involves: BALB/cJ * C57BL/6NCrlj * CBA/JNCrlj	MGI:6367553

Genotype
MGI:6367552

hm1

• Allelic Composition: Ociad1^tm1.1Msin/Ociad1^tm1.1Msin
• Genetic Background: involves: BALB/cJ * C57BL/6NCrlj * CBA/JNCrlj

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

increased mortality induced by gamma-irradiation ( J:279239 )

• about 50% of mice irradiated with a sublethal dose (6.5 Gy) of gamma irradiation die within 15 days while all controls survive beyond 25 days

increased susceptibility to xenobiotic induced morbidity/mortality ( J:279239 )

• increase in mortality following weekly injections of 150 mg/kg of 5-FU for 3 weeks compared to similarly treated controls

premature death ( J:279239 )

• decreased lifespan

hematopoietic system

enlarged spleen ( J:279239 )

• by 12 months of age

abnormal bone marrow cell number ( J:279239 )

• increase in the CD11b+ myeloid fraction

decreased bone marrow cell number ( J:279239 )

• decrease in the MPP3 (LSK-CD150^-CD48+) cell population

increased bone marrow cell number ( J:279239 )

• increase in cellularity at 2 and 6 months of age

increased granulocyte monocyte progenitor cell number ( J:279239 )

• increase in percentage and absolute numbers

decreased common lymphocyte progenitor cell number ( J:279239 )

• decrease in common lymphoid progenitors and CD19+ lymphoid fraction

decreased B cell number ( J:279239 )

• decreased percentage of B cells at 6 months of age

decreased T cell number ( J:279239 )

• decreased percentage of T cells at 6 months of age

increased hematopoietic cell number ( J:279239 )

• increase in peripheral blood cell numbers at 2 4 months of age with a progressive increase in numbers with age

increased myeloid cell number in bone marrow ( J:279239 )

• increase in percentages and absolute numbers of myeloid-enriched progenitor populations

• myeloid infiltration of femurs by 2 months of age

increased granulocyte number ( J:279239 )

• increased percentage of granulocytes at 6 months of age

increased neutrophil cell number ( J:279239 )

• increased percentage of neutrophils at 6 months of age

increased macrophage cell number ( J:279239 )

• increased percentage of macrophages at 6 months of age

increased monocyte cell number ( J:279239 )

• increased percentage of monocytes at 6 months of age

increased spleen germinal center size ( J:279239 )

• enlarged follicles at 12 months of age

abnormal hematopoietic stem cell physiology ( J:279239 )

• increased accumulation of DNA damage and decreased apoptosis in hematopoietic stem cells

increased hematopoietic stem cell proliferation ( J:279239 )

• in culture cells form larger colonies and a greater number of colonies

• treatment with Nutlin-3, an MDM2 antagonist, inhibits the expansion of hematopoietic stem cells

homeostasis/metabolism

abnormal response to radiation ( J:279239 )

• at 7 days after irradiation show significantly lower peripheral blood cells counts compared to similarly treated controls

• at 12 days post irradiation hematopoietic stem cell percentages are significantly lower compared to similarly treated controls

increased mortality induced by gamma-irradiation ( J:279239 )

• about 50% of mice irradiated with a sublethal dose (6.5 Gy) of gamma irradiation die within 15 days while all controls survive beyond 25 days

increased susceptibility to xenobiotic induced morbidity/mortality ( J:279239 )

• increase in mortality following weekly injections of 150 mg/kg of 5-FU for 3 weeks compared to similarly treated controls

increased physiological sensitivity to xenobiotic ( J:279239 )

• greater decrease in peripheral blood cell numbers following weekly injections of 150 mg/kg of 5-FU for 3 weeks compared to similarly treated controls

liver/biliary system

abnormal liver morphology ( J:279239 )

• infiltration of myeloid cells at 12 months of age

renal/urinary system

abnormal kidney morphology ( J:279239 )

• infiltration of myeloid cells at 12 months of age

skeleton

kyphosis ( J:279239 )

• prone to developing kyphosis

cellular

decreased apoptosis ( J:279239 )

• decreased apoptosis of hematopoietic stem cells both in vivo and after short term culture

increased hematopoietic stem cell proliferation ( J:279239 )

• in culture cells form larger colonies and a greater number of colonies

• treatment with Nutlin-3, an MDM2 antagonist, inhibits the expansion of hematopoietic stem cells

immune system

enlarged spleen ( J:279239 )

• by 12 months of age

increased granulocyte number ( J:279239 )

• increased percentage of granulocytes at 6 months of age

increased neutrophil cell number ( J:279239 )

• increased percentage of neutrophils at 6 months of age

decreased B cell number ( J:279239 )

• decreased percentage of B cells at 6 months of age

decreased T cell number ( J:279239 )

• decreased percentage of T cells at 6 months of age

increased macrophage cell number ( J:279239 )

• increased percentage of macrophages at 6 months of age

increased monocyte cell number ( J:279239 )

• increased percentage of monocytes at 6 months of age

increased spleen germinal center size ( J:279239 )

• enlarged follicles at 12 months of age

growth/size/body

enlarged spleen ( J:279239 )

• by 12 months of age

Genotype
MGI:6367553

ht2

• Allelic Composition: Ociad1^tm1.1Msin/Ociad1⁺
• Genetic Background: involves: BALB/cJ * C57BL/6NCrlj * CBA/JNCrlj

hematopoietic system

increased erythrocyte cell number ( J:279239 )

• modest increase in red blood cell numbers at 6 months of age

abnormal platelet cell number ( J:279239 )

• modest increase in platelet numbers at 6 months of age